Epigenetic programming of adipose-derived stem cells in low birthweight individuals
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Epigenetic programming of adipose-derived stem cells in low birthweight individuals. / Broholm, Christa; Olsson, Anders H; Perfilyev, Alexander; Hansen, Ninna S; Schrölkamp, Maren; Strasko, Klaudia S.; Scheele, Camilla; Ribel-Madsen, Rasmus; Mortensen, Brynjulf; Jørgensen, Sine W.; Ling, Charlotte; Vaag, Allan.
I: Diabetologia, Bind 59, Nr. 12, 2016, s. 2664-2673.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Epigenetic programming of adipose-derived stem cells in low birthweight individuals
AU - Broholm, Christa
AU - Olsson, Anders H
AU - Perfilyev, Alexander
AU - Hansen, Ninna S
AU - Schrölkamp, Maren
AU - Strasko, Klaudia S.
AU - Scheele, Camilla
AU - Ribel-Madsen, Rasmus
AU - Mortensen, Brynjulf
AU - Jørgensen, Sine W.
AU - Ling, Charlotte
AU - Vaag, Allan
PY - 2016
Y1 - 2016
N2 - Aims/hypothesis: Low birthweight (LBW) is associated with dysfunctions of adipose tissue and metabolic disease in adult life. We hypothesised that altered epigenetic and transcriptional regulation of adipose-derived stem cells (ADSCs) could play a role in programming adipose tissue dysfunction in LBW individuals. Methods: ADSCs were isolated from the subcutaneous adipose tissue of 13 normal birthweight (NBW) and 13 LBW adult men. The adipocytes were cultured in vitro, and genome-wide differences in RNA expression and DNA methylation profiles were analysed in ADSCs and differentiated adipocytes. Results: We demonstrated that ADSCs from LBW individuals exhibit multiple expression changes as well as genome-wide alterations in methylation pattern. Reduced expression of the transcription factor cyclin T2 encoded by CCNT2 may play a key role in orchestrating several of the gene expression changes in ADSCs from LBW individuals. Indeed, silencing of CCNT2 in human adipocytes decreased leptin secretion as well as the mRNA expression of several genes involved in adipogenesis, including MGLL, LIPE, PPARG, LEP and ADIPOQ. Only subtle genome-wide mRNA expression and DNA methylation changes were seen in mature cultured adipocytes from LBW individuals. Conclusions/interpretation: Epigenetic and transcriptional changes in LBW individuals are most pronounced in immature ADSCs that in turn may programme physiological characteristics of the mature adipocytes that influence the risk of metabolic diseases. Reduced expression of CCNT2 may play a key role in the developmental programming of adipose tissue.
AB - Aims/hypothesis: Low birthweight (LBW) is associated with dysfunctions of adipose tissue and metabolic disease in adult life. We hypothesised that altered epigenetic and transcriptional regulation of adipose-derived stem cells (ADSCs) could play a role in programming adipose tissue dysfunction in LBW individuals. Methods: ADSCs were isolated from the subcutaneous adipose tissue of 13 normal birthweight (NBW) and 13 LBW adult men. The adipocytes were cultured in vitro, and genome-wide differences in RNA expression and DNA methylation profiles were analysed in ADSCs and differentiated adipocytes. Results: We demonstrated that ADSCs from LBW individuals exhibit multiple expression changes as well as genome-wide alterations in methylation pattern. Reduced expression of the transcription factor cyclin T2 encoded by CCNT2 may play a key role in orchestrating several of the gene expression changes in ADSCs from LBW individuals. Indeed, silencing of CCNT2 in human adipocytes decreased leptin secretion as well as the mRNA expression of several genes involved in adipogenesis, including MGLL, LIPE, PPARG, LEP and ADIPOQ. Only subtle genome-wide mRNA expression and DNA methylation changes were seen in mature cultured adipocytes from LBW individuals. Conclusions/interpretation: Epigenetic and transcriptional changes in LBW individuals are most pronounced in immature ADSCs that in turn may programme physiological characteristics of the mature adipocytes that influence the risk of metabolic diseases. Reduced expression of CCNT2 may play a key role in the developmental programming of adipose tissue.
KW - Adipocytes
KW - Adipogenesis
KW - Adipose tissue
KW - Cyclin T2
KW - Epigenetics
KW - Fetal programming
KW - Low birthweight
KW - Metabolic disease
KW - Type 2 diabetes
U2 - 10.1007/s00125-016-4099-9
DO - 10.1007/s00125-016-4099-9
M3 - Journal article
C2 - 27627980
AN - SCOPUS:84987618034
VL - 59
SP - 2664
EP - 2673
JO - Diabetologia
JF - Diabetologia
SN - 0012-186X
IS - 12
ER -
ID: 179054493