Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes

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Standard

Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes. / Ghavami, Mahdi; Shiraishi, Takehiko; Nielsen, Peter E.

I: ACS Applied Bio Materials, Bind 3, Nr. 2, 2020, s. 1018-1025.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ghavami, M, Shiraishi, T & Nielsen, PE 2020, 'Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes', ACS Applied Bio Materials, bind 3, nr. 2, s. 1018-1025. https://doi.org/10.1021/acsabm.9b01022

APA

Ghavami, M., Shiraishi, T., & Nielsen, P. E. (2020). Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes. ACS Applied Bio Materials, 3(2), 1018-1025. https://doi.org/10.1021/acsabm.9b01022

Vancouver

Ghavami M, Shiraishi T, Nielsen PE. Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes. ACS Applied Bio Materials. 2020;3(2):1018-1025. https://doi.org/10.1021/acsabm.9b01022

Author

Ghavami, Mahdi ; Shiraishi, Takehiko ; Nielsen, Peter E. / Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes. I: ACS Applied Bio Materials. 2020 ; Bind 3, Nr. 2. s. 1018-1025.

Bibtex

@article{6309e9de39344c4caeaef47959a6f795,
title = "Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes",
abstract = "Phospholipase sensitive liposomes (PSLs) have attracted great attention in targeted anticancer drug delivery due to cargo release triggered by tumor-secreted phospholipase A2 (sPLA2). Such liposomes could also serve as a vehicle for tissue-specific delivery of antisense therapeutics to (solid) tumors. While extensive studies on developing PSL formulations for small molecules exist, hardly any data are available on delivering larger molecules such as antisense agents. The present study demonstrates PSL encapsulation and phospholipase A2 triggered the release of a splice correcting, antisense octaarginine-peptide nucleic acid (octaarginine-PNA) conjugate. The results show that, although PNA can be efficiently encapsulated in PSL and also released using sPLA2 in serum-free conditions, the release is inhibited in the presence of serum. This is ascribed to the adsorption of serum proteins, including serum albumin and apolipoprotein C-III, to the surface of PSL (corona formation) and consequent prevention of sPLA2-mediated PNA release.",
keywords = "antisense PNA, drug delivery, liposomes, phospholipase A2, phospholipase-triggered release, protein corona",
author = "Mahdi Ghavami and Takehiko Shiraishi and Nielsen, {Peter E.}",
year = "2020",
doi = "10.1021/acsabm.9b01022",
language = "English",
volume = "3",
pages = "1018--1025",
journal = "ACS Applied Bio Materials",
issn = "2576-6422",
publisher = "American Chemical Society",
number = "2",

}

RIS

TY - JOUR

T1 - Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes

AU - Ghavami, Mahdi

AU - Shiraishi, Takehiko

AU - Nielsen, Peter E.

PY - 2020

Y1 - 2020

N2 - Phospholipase sensitive liposomes (PSLs) have attracted great attention in targeted anticancer drug delivery due to cargo release triggered by tumor-secreted phospholipase A2 (sPLA2). Such liposomes could also serve as a vehicle for tissue-specific delivery of antisense therapeutics to (solid) tumors. While extensive studies on developing PSL formulations for small molecules exist, hardly any data are available on delivering larger molecules such as antisense agents. The present study demonstrates PSL encapsulation and phospholipase A2 triggered the release of a splice correcting, antisense octaarginine-peptide nucleic acid (octaarginine-PNA) conjugate. The results show that, although PNA can be efficiently encapsulated in PSL and also released using sPLA2 in serum-free conditions, the release is inhibited in the presence of serum. This is ascribed to the adsorption of serum proteins, including serum albumin and apolipoprotein C-III, to the surface of PSL (corona formation) and consequent prevention of sPLA2-mediated PNA release.

AB - Phospholipase sensitive liposomes (PSLs) have attracted great attention in targeted anticancer drug delivery due to cargo release triggered by tumor-secreted phospholipase A2 (sPLA2). Such liposomes could also serve as a vehicle for tissue-specific delivery of antisense therapeutics to (solid) tumors. While extensive studies on developing PSL formulations for small molecules exist, hardly any data are available on delivering larger molecules such as antisense agents. The present study demonstrates PSL encapsulation and phospholipase A2 triggered the release of a splice correcting, antisense octaarginine-peptide nucleic acid (octaarginine-PNA) conjugate. The results show that, although PNA can be efficiently encapsulated in PSL and also released using sPLA2 in serum-free conditions, the release is inhibited in the presence of serum. This is ascribed to the adsorption of serum proteins, including serum albumin and apolipoprotein C-III, to the surface of PSL (corona formation) and consequent prevention of sPLA2-mediated PNA release.

KW - antisense PNA

KW - drug delivery

KW - liposomes

KW - phospholipase A2

KW - phospholipase-triggered release

KW - protein corona

U2 - 10.1021/acsabm.9b01022

DO - 10.1021/acsabm.9b01022

M3 - Journal article

AN - SCOPUS:85080089988

VL - 3

SP - 1018

EP - 1025

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

SN - 2576-6422

IS - 2

ER -

ID: 237413914