Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors

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Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors. / Pundhir, Sachin; Bratt Lauridsen, Felicia Kathrine; Schuster, Mikkel Bruhn; Jakobsen, Janus Schou; Ge, Ying; Schoof, Erwin Marten; Rapin, Nicolas Philippe Jean-Pierre; Waage, Johannes; Hasemann, Marie Sigurd; Porse, Bo Torben.

I: Cell Reports, Bind 23, Nr. 9, 2018, s. 2744-2757.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Pundhir, S, Bratt Lauridsen, FK, Schuster, MB, Jakobsen, JS, Ge, Y, Schoof, EM, Rapin, NPJ-P, Waage, J, Hasemann, MS & Porse, BT 2018, 'Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors', Cell Reports, bind 23, nr. 9, s. 2744-2757. https://doi.org/10.1016/j.celrep.2018.05.012

APA

Pundhir, S., Bratt Lauridsen, F. K., Schuster, M. B., Jakobsen, J. S., Ge, Y., Schoof, E. M., Rapin, N. P. J-P., Waage, J., Hasemann, M. S., & Porse, B. T. (2018). Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors. Cell Reports, 23(9), 2744-2757. https://doi.org/10.1016/j.celrep.2018.05.012

Vancouver

Pundhir S, Bratt Lauridsen FK, Schuster MB, Jakobsen JS, Ge Y, Schoof EM o.a. Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors. Cell Reports. 2018;23(9):2744-2757. https://doi.org/10.1016/j.celrep.2018.05.012

Author

Pundhir, Sachin ; Bratt Lauridsen, Felicia Kathrine ; Schuster, Mikkel Bruhn ; Jakobsen, Janus Schou ; Ge, Ying ; Schoof, Erwin Marten ; Rapin, Nicolas Philippe Jean-Pierre ; Waage, Johannes ; Hasemann, Marie Sigurd ; Porse, Bo Torben. / Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors. I: Cell Reports. 2018 ; Bind 23, Nr. 9. s. 2744-2757.

Bibtex

@article{4feb0e956e73495896ff9f6f7a2c7589,

title = "Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors",

abstract = "Transcription factors PU.1 and CEBPA are required for the proper coordination of enhancer activity during granulocytic-monocytic (GM) lineage differentiation to form myeloid cells. However, precisely how these factors control the chronology of enhancer establishment during differentiation is not known. Through integrated analyses of enhancer dynamics, transcription factor binding, and proximal gene expression during successive stages of murine GM-lineage differentiation, we unravel the distinct kinetics by which PU.1 and CEBPA coordinate GM enhancer activity. We find no evidence of a pioneering function of PU.1 during late GM-lineage differentiation. Instead, we delineate a set of enhancers that gain accessibility in a CEBPA-dependent manner, suggesting a pioneering function of CEBPA. Analyses of Cebpa null bone marrow demonstrate that CEBPA controls PU.1 levels and, unexpectedly, that the loss of CEBPA results in an early differentiation block. Taken together, our data provide insights into how PU.1 and CEBPA functionally interact to drive GM-lineage differentiation.",

author = "Sachin Pundhir and {Bratt Lauridsen}, {Felicia Kathrine} and Schuster, {Mikkel Bruhn} and Jakobsen, {Janus Schou} and Ying Ge and Schoof, {Erwin Marten} and Rapin, {Nicolas Philippe Jean-Pierre} and Johannes Waage and Hasemann, {Marie Sigurd} and Porse, {Bo Torben}",

year = "2018",

doi = "10.1016/j.celrep.2018.05.012",

language = "English",

volume = "23",

pages = "2744--2757",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "9",

}

RIS

TY - JOUR

T1 - Enhancer and Transcription Factor Dynamics during Myeloid Differentiation Reveal an Early Differentiation Block in Cebpa null Progenitors

AU - Pundhir, Sachin

AU - Bratt Lauridsen, Felicia Kathrine

AU - Schuster, Mikkel Bruhn

AU - Jakobsen, Janus Schou

AU - Ge, Ying

AU - Schoof, Erwin Marten

AU - Rapin, Nicolas Philippe Jean-Pierre

AU - Waage, Johannes

AU - Hasemann, Marie Sigurd

AU - Porse, Bo Torben

PY - 2018

Y1 - 2018

N2 - Transcription factors PU.1 and CEBPA are required for the proper coordination of enhancer activity during granulocytic-monocytic (GM) lineage differentiation to form myeloid cells. However, precisely how these factors control the chronology of enhancer establishment during differentiation is not known. Through integrated analyses of enhancer dynamics, transcription factor binding, and proximal gene expression during successive stages of murine GM-lineage differentiation, we unravel the distinct kinetics by which PU.1 and CEBPA coordinate GM enhancer activity. We find no evidence of a pioneering function of PU.1 during late GM-lineage differentiation. Instead, we delineate a set of enhancers that gain accessibility in a CEBPA-dependent manner, suggesting a pioneering function of CEBPA. Analyses of Cebpa null bone marrow demonstrate that CEBPA controls PU.1 levels and, unexpectedly, that the loss of CEBPA results in an early differentiation block. Taken together, our data provide insights into how PU.1 and CEBPA functionally interact to drive GM-lineage differentiation.

AB - Transcription factors PU.1 and CEBPA are required for the proper coordination of enhancer activity during granulocytic-monocytic (GM) lineage differentiation to form myeloid cells. However, precisely how these factors control the chronology of enhancer establishment during differentiation is not known. Through integrated analyses of enhancer dynamics, transcription factor binding, and proximal gene expression during successive stages of murine GM-lineage differentiation, we unravel the distinct kinetics by which PU.1 and CEBPA coordinate GM enhancer activity. We find no evidence of a pioneering function of PU.1 during late GM-lineage differentiation. Instead, we delineate a set of enhancers that gain accessibility in a CEBPA-dependent manner, suggesting a pioneering function of CEBPA. Analyses of Cebpa null bone marrow demonstrate that CEBPA controls PU.1 levels and, unexpectedly, that the loss of CEBPA results in an early differentiation block. Taken together, our data provide insights into how PU.1 and CEBPA functionally interact to drive GM-lineage differentiation.

U2 - 10.1016/j.celrep.2018.05.012

DO - 10.1016/j.celrep.2018.05.012

M3 - Journal article

C2 - 29847803

VL - 23

SP - 2744

EP - 2757

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 9

ER -

ID: 199464659