Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood

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Standard

Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood. / Nurhasan, Mulia; Roos, Nanna; Aristizabal Henao, J J; Chamnan, C; Stark, K D; Lauritzen, Lotte.

I: Prostaglandins, Leukotrienes & Essential Fatty Acids, Bind 96, Nr. 1, 2015, s. 57-61.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nurhasan, M, Roos, N, Aristizabal Henao, JJ, Chamnan, C, Stark, KD & Lauritzen, L 2015, 'Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood', Prostaglandins, Leukotrienes & Essential Fatty Acids, bind 96, nr. 1, s. 57-61. https://doi.org/10.1016/j.plefa.2015.02.001

APA

Nurhasan, M., Roos, N., Aristizabal Henao, J. J., Chamnan, C., Stark, K. D., & Lauritzen, L. (2015). Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood. Prostaglandins, Leukotrienes & Essential Fatty Acids, 96(1), 57-61. https://doi.org/10.1016/j.plefa.2015.02.001

Vancouver

Nurhasan M, Roos N, Aristizabal Henao JJ, Chamnan C, Stark KD, Lauritzen L. Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood. Prostaglandins, Leukotrienes & Essential Fatty Acids. 2015;96(1):57-61. https://doi.org/10.1016/j.plefa.2015.02.001

Author

Nurhasan, Mulia ; Roos, Nanna ; Aristizabal Henao, J J ; Chamnan, C ; Stark, K D ; Lauritzen, Lotte. / Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood. I: Prostaglandins, Leukotrienes & Essential Fatty Acids. 2015 ; Bind 96, Nr. 1. s. 57-61.

Bibtex

@article{2089911961434b53b89f7d49c942c0d0,
title = "Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood",
abstract = "Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20°C and -80°C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20°C and -80°C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8±2.7 vs. 14.4±2.5%, p<0.001) and a lower n-6/n-3 ratio (6.4±1.4 vs. 6.9±1.4, p<0.001) in the -20°C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.",
author = "Mulia Nurhasan and Nanna Roos and {Aristizabal Henao}, {J J} and C Chamnan and Stark, {K D} and Lotte Lauritzen",
note = "CURIS 2015 NEXS 098",
year = "2015",
doi = "10.1016/j.plefa.2015.02.001",
language = "English",
volume = "96",
pages = "57--61",
journal = "Prostaglandins, Leukotrienes & Essential Fatty Acids",
issn = "0952-3278",
publisher = "Churchill Livingstone",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood

AU - Nurhasan, Mulia

AU - Roos, Nanna

AU - Aristizabal Henao, J J

AU - Chamnan, C

AU - Stark, K D

AU - Lauritzen, Lotte

N1 - CURIS 2015 NEXS 098

PY - 2015

Y1 - 2015

N2 - Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20°C and -80°C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20°C and -80°C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8±2.7 vs. 14.4±2.5%, p<0.001) and a lower n-6/n-3 ratio (6.4±1.4 vs. 6.9±1.4, p<0.001) in the -20°C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.

AB - Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20°C and -80°C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20°C and -80°C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8±2.7 vs. 14.4±2.5%, p<0.001) and a lower n-6/n-3 ratio (6.4±1.4 vs. 6.9±1.4, p<0.001) in the -20°C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.

U2 - 10.1016/j.plefa.2015.02.001

DO - 10.1016/j.plefa.2015.02.001

M3 - Journal article

C2 - 25753812

VL - 96

SP - 57

EP - 61

JO - Prostaglandins, Leukotrienes & Essential Fatty Acids

JF - Prostaglandins, Leukotrienes & Essential Fatty Acids

SN - 0952-3278

IS - 1

ER -

ID: 132474793