Do senescence markers correlate in vitro and in situ within individual human donors?

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Standard

Do senescence markers correlate in vitro and in situ within individual human donors? / Waaijer, Mariëtte E C; Gunn, David A; van Heemst, Diana; Slagboom, P Eline; Sedivy, John M; Dirks, Roeland W; Tanke, Hans J; Westendorp, Rudi G J; Maier, Andrea B.

I: Aging, Bind 10, Nr. 2, 2018, s. 278-289.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Waaijer, MEC, Gunn, DA, van Heemst, D, Slagboom, PE, Sedivy, JM, Dirks, RW, Tanke, HJ, Westendorp, RGJ & Maier, AB 2018, 'Do senescence markers correlate in vitro and in situ within individual human donors?', Aging, bind 10, nr. 2, s. 278-289. https://doi.org/10.18632/aging.101389

APA

Waaijer, M. E. C., Gunn, D. A., van Heemst, D., Slagboom, P. E., Sedivy, J. M., Dirks, R. W., Tanke, H. J., Westendorp, R. G. J., & Maier, A. B. (2018). Do senescence markers correlate in vitro and in situ within individual human donors? Aging, 10(2), 278-289. https://doi.org/10.18632/aging.101389

Vancouver

Waaijer MEC, Gunn DA, van Heemst D, Slagboom PE, Sedivy JM, Dirks RW o.a. Do senescence markers correlate in vitro and in situ within individual human donors? Aging. 2018;10(2):278-289. https://doi.org/10.18632/aging.101389

Author

Waaijer, Mariëtte E C ; Gunn, David A ; van Heemst, Diana ; Slagboom, P Eline ; Sedivy, John M ; Dirks, Roeland W ; Tanke, Hans J ; Westendorp, Rudi G J ; Maier, Andrea B. / Do senescence markers correlate in vitro and in situ within individual human donors?. I: Aging. 2018 ; Bind 10, Nr. 2. s. 278-289.

Bibtex

@article{42e95e7b5e384c96b95cacd71e8f87fe,
title = "Do senescence markers correlate in vitro and in situ within individual human donors?",
abstract = "Little is known on how well senescence markers in vitro and in situ correlate within individual donors. We studied correlations between the same and different in vitro markers. Furthermore, we tested correlations between in vitro markers with in situ p16INK4a positivity.From 100 donors (20-91 years), cultured dermal fibroblasts were assessed for reactive oxygen species (ROS), telomere-associated foci (TAF), p16INK4a and senescence-associated β-gal (SAβ-gal), with/ without 0.6 µM rotenone for 3 days (short-term). In fibroblasts from 40 donors, telomere shortening, ROS and SAβ-gal were additionally assessed, with/ without 20 nM rotenone for 7 weeks (long-term). In skin from 52 donors, the number of p16INK4a positive dermal cells was assessed in situ.More than half of the correlations of the same senescence markers in vitro between duplicate experiments and between short-term versus long-term experiments were significant. Half of the different senescence marker correlations were significant within the short-term and within the long-term experiments. The different senescence markers in vitro were not significantly correlated intra-individually with in situ p16INK4a positivity.
In conclusion, the same and different senescence markers are frequently correlated significantly within and between in vitro experiments, but in vitro senescence markers are not correlated with p16INK4a positivity in situ.",
author = "Waaijer, {Mari{\"e}tte E C} and Gunn, {David A} and {van Heemst}, Diana and Slagboom, {P Eline} and Sedivy, {John M} and Dirks, {Roeland W} and Tanke, {Hans J} and Westendorp, {Rudi G J} and Maier, {Andrea B.}",
year = "2018",
doi = "10.18632/aging.101389",
language = "English",
volume = "10",
pages = "278--289",
journal = "Aging",
issn = "1945-4589",
publisher = "Impact Journals LLC",
number = "2",

}

RIS

TY - JOUR

T1 - Do senescence markers correlate in vitro and in situ within individual human donors?

AU - Waaijer, Mariëtte E C

AU - Gunn, David A

AU - van Heemst, Diana

AU - Slagboom, P Eline

AU - Sedivy, John M

AU - Dirks, Roeland W

AU - Tanke, Hans J

AU - Westendorp, Rudi G J

AU - Maier, Andrea B.

PY - 2018

Y1 - 2018

N2 - Little is known on how well senescence markers in vitro and in situ correlate within individual donors. We studied correlations between the same and different in vitro markers. Furthermore, we tested correlations between in vitro markers with in situ p16INK4a positivity.From 100 donors (20-91 years), cultured dermal fibroblasts were assessed for reactive oxygen species (ROS), telomere-associated foci (TAF), p16INK4a and senescence-associated β-gal (SAβ-gal), with/ without 0.6 µM rotenone for 3 days (short-term). In fibroblasts from 40 donors, telomere shortening, ROS and SAβ-gal were additionally assessed, with/ without 20 nM rotenone for 7 weeks (long-term). In skin from 52 donors, the number of p16INK4a positive dermal cells was assessed in situ.More than half of the correlations of the same senescence markers in vitro between duplicate experiments and between short-term versus long-term experiments were significant. Half of the different senescence marker correlations were significant within the short-term and within the long-term experiments. The different senescence markers in vitro were not significantly correlated intra-individually with in situ p16INK4a positivity.
In conclusion, the same and different senescence markers are frequently correlated significantly within and between in vitro experiments, but in vitro senescence markers are not correlated with p16INK4a positivity in situ.

AB - Little is known on how well senescence markers in vitro and in situ correlate within individual donors. We studied correlations between the same and different in vitro markers. Furthermore, we tested correlations between in vitro markers with in situ p16INK4a positivity.From 100 donors (20-91 years), cultured dermal fibroblasts were assessed for reactive oxygen species (ROS), telomere-associated foci (TAF), p16INK4a and senescence-associated β-gal (SAβ-gal), with/ without 0.6 µM rotenone for 3 days (short-term). In fibroblasts from 40 donors, telomere shortening, ROS and SAβ-gal were additionally assessed, with/ without 20 nM rotenone for 7 weeks (long-term). In skin from 52 donors, the number of p16INK4a positive dermal cells was assessed in situ.More than half of the correlations of the same senescence markers in vitro between duplicate experiments and between short-term versus long-term experiments were significant. Half of the different senescence marker correlations were significant within the short-term and within the long-term experiments. The different senescence markers in vitro were not significantly correlated intra-individually with in situ p16INK4a positivity.
In conclusion, the same and different senescence markers are frequently correlated significantly within and between in vitro experiments, but in vitro senescence markers are not correlated with p16INK4a positivity in situ.

U2 - 10.18632/aging.101389

DO - 10.18632/aging.101389

M3 - Journal article

C2 - 29500330

VL - 10

SP - 278

EP - 289

JO - Aging

JF - Aging

SN - 1945-4589

IS - 2

ER -

ID: 195463325