DNA damage invokes mitophagy through a pathway involving Spata18
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DNA damage invokes mitophagy through a pathway involving Spata18. / Dan, Xiuli; Babbar, Mansi; Moore, Anthony; Wechter, Noah; Tian, Jingyan; Mohanty, Joy G; Croteau, Deborah L; Bohr, Vilhelm A.
I: Nucleic Acids Research, Bind 48, Nr. 12, 2020, s. 6611-6623.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - DNA damage invokes mitophagy through a pathway involving Spata18
AU - Dan, Xiuli
AU - Babbar, Mansi
AU - Moore, Anthony
AU - Wechter, Noah
AU - Tian, Jingyan
AU - Mohanty, Joy G
AU - Croteau, Deborah L
AU - Bohr, Vilhelm A
N1 - Published by Oxford University Press on behalf of Nucleic Acids Research 2020.
PY - 2020
Y1 - 2020
N2 - Mitochondria are vital for cellular energy supply and intracellular signaling after stress. Here, we aimed to investigate how mitochondria respond to acute DNA damage with respect to mitophagy, which is an important mitochondrial quality control process. Our results show that mitophagy increases after DNA damage in primary fibroblasts, murine neurons and Caenorhabditis elegans neurons. Our results indicate that modulation of mitophagy after DNA damage is independent of the type of DNA damage stimuli used and that the protein Spata18 is an important player in this process. Knockdown of Spata18 suppresses mitophagy, disturbs mitochondrial Ca2+ homeostasis, affects ATP production, and attenuates DNA repair. Importantly, mitophagy after DNA damage is a vital cellular response to maintain mitochondrial functions and DNA repair.
AB - Mitochondria are vital for cellular energy supply and intracellular signaling after stress. Here, we aimed to investigate how mitochondria respond to acute DNA damage with respect to mitophagy, which is an important mitochondrial quality control process. Our results show that mitophagy increases after DNA damage in primary fibroblasts, murine neurons and Caenorhabditis elegans neurons. Our results indicate that modulation of mitophagy after DNA damage is independent of the type of DNA damage stimuli used and that the protein Spata18 is an important player in this process. Knockdown of Spata18 suppresses mitophagy, disturbs mitochondrial Ca2+ homeostasis, affects ATP production, and attenuates DNA repair. Importantly, mitophagy after DNA damage is a vital cellular response to maintain mitochondrial functions and DNA repair.
KW - Animals
KW - Caenorhabditis elegans/genetics
KW - Calcium/metabolism
KW - Cell Line
KW - Cell Proliferation/genetics
KW - DNA Damage/genetics
KW - DNA Repair/genetics
KW - Fibroblasts/metabolism
KW - Humans
KW - Mice
KW - Mitochondria/genetics
KW - Mitochondrial Proteins/genetics
KW - Mitophagy/genetics
KW - Neurons/metabolism
U2 - 10.1093/nar/gkaa393
DO - 10.1093/nar/gkaa393
M3 - Journal article
C2 - 32453416
VL - 48
SP - 6611
EP - 6623
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 12
ER -
ID: 257866027