Diarrhoea-causing microorganisms are rare in adult patients undergoing diagnostic laparoscopy for suspected appendicitis: a prospective observational cohort study
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Diarrhoea-causing microorganisms are rare in adult patients undergoing diagnostic laparoscopy for suspected appendicitis : a prospective observational cohort study. / Fonnes, Siv; Rasmussen, Tilde; Holzknecht, Barbara Juliane; Olesen, Christoffer Skov; Olsen, Joachim Hjalde Halmsted; Schmidt, Line; Alder, Rasmus; Sørensen, Sara Gamborg; Gülen, Sengül; Klarskov, Louise Laurberg; Krogfelt, Karen Angeliki; Westh, Henrik; Rosenberg, Jacob.
I: APMIS, Bind 131, Nr. 6, 2023, s. 284-293.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Diarrhoea-causing microorganisms are rare in adult patients undergoing diagnostic laparoscopy for suspected appendicitis
T2 - a prospective observational cohort study
AU - Fonnes, Siv
AU - Rasmussen, Tilde
AU - Holzknecht, Barbara Juliane
AU - Olesen, Christoffer Skov
AU - Olsen, Joachim Hjalde Halmsted
AU - Schmidt, Line
AU - Alder, Rasmus
AU - Sørensen, Sara Gamborg
AU - Gülen, Sengül
AU - Klarskov, Louise Laurberg
AU - Krogfelt, Karen Angeliki
AU - Westh, Henrik
AU - Rosenberg, Jacob
N1 - Publisher Copyright: © 2023 Scandinavian Societies for Pathology, Medical Microbiology and Immunology.
PY - 2023
Y1 - 2023
N2 - We investigated if diarrhoea-causing bacteria, including Yersinia species, could mimic the symptoms of appendicitis and lead to surgery. This prospective observational cohort study (NCT03349814) included adult patients undergoing surgery for suspected appendicitis. Rectal swabs were analysed with polymerase chain reaction (PCR) for Yersinia, Campylobacter, Salmonella, Shigella and Aeromonas spp. Blood samples were analysed routinely and with an in-house ELISA serological test for Yersinia enterocolitica antibodies. We compared patients without appendicitis and patients with appendicitis confirmed by histopathology. The outcomes included PCR-confirmed infection with Yersinia spp., serologic-confirmed infection with Y. enterocolitica, PCR-confirmed infection with other diarrhoea-causing bacteria and Enterobius vermicularis confirmed by histopathology. A total of 224 patients were included, 51 without and 173 with appendicitis, and followed for 10 days. PCR-confirmed infection with Yersinia spp. was found in one patient (2%) without appendicitis and no patients (0%) with appendicitis (p = 0.23). Serology was positive for Y. enterocolitica for the same patient without appendicitis and two patients with appendicitis (p = 0.54). Campylobacter spp. were detected in 4% vs 1% (p = 0.13) of patients without and with appendicitis, respectively. Infection with Yersinia spp. and other diarrhoea-causing microorganisms in adult patients undergoing surgery for suspected appendicitis was rare.
AB - We investigated if diarrhoea-causing bacteria, including Yersinia species, could mimic the symptoms of appendicitis and lead to surgery. This prospective observational cohort study (NCT03349814) included adult patients undergoing surgery for suspected appendicitis. Rectal swabs were analysed with polymerase chain reaction (PCR) for Yersinia, Campylobacter, Salmonella, Shigella and Aeromonas spp. Blood samples were analysed routinely and with an in-house ELISA serological test for Yersinia enterocolitica antibodies. We compared patients without appendicitis and patients with appendicitis confirmed by histopathology. The outcomes included PCR-confirmed infection with Yersinia spp., serologic-confirmed infection with Y. enterocolitica, PCR-confirmed infection with other diarrhoea-causing bacteria and Enterobius vermicularis confirmed by histopathology. A total of 224 patients were included, 51 without and 173 with appendicitis, and followed for 10 days. PCR-confirmed infection with Yersinia spp. was found in one patient (2%) without appendicitis and no patients (0%) with appendicitis (p = 0.23). Serology was positive for Y. enterocolitica for the same patient without appendicitis and two patients with appendicitis (p = 0.54). Campylobacter spp. were detected in 4% vs 1% (p = 0.13) of patients without and with appendicitis, respectively. Infection with Yersinia spp. and other diarrhoea-causing microorganisms in adult patients undergoing surgery for suspected appendicitis was rare.
KW - appendicitis
KW - clinical trial
KW - PCR
KW - serology
KW - yersinia
U2 - 10.1111/apm.13311
DO - 10.1111/apm.13311
M3 - Journal article
C2 - 36932839
AN - SCOPUS:85151462435
VL - 131
SP - 284
EP - 293
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 6
ER -
ID: 359805606