Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000

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Standard

Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000. / Farzad, Maryam Sharafi; Pedersen, Brian Møllegaard; Mogensen, Helle Smidt; Børsting, Claus.

I: BioTechniques, Bind 68, Nr. 6, 01.06.2020, s. 342-344.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Farzad, MS, Pedersen, BM, Mogensen, HS & Børsting, C 2020, 'Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000', BioTechniques, bind 68, nr. 6, s. 342-344. https://doi.org/10.2144/btn-2019-0156

APA

Farzad, M. S., Pedersen, B. M., Mogensen, H. S., & Børsting, C. (2020). Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000. BioTechniques, 68(6), 342-344. https://doi.org/10.2144/btn-2019-0156

Vancouver

Farzad MS, Pedersen BM, Mogensen HS, Børsting C. Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000. BioTechniques. 2020 jun. 1;68(6):342-344. https://doi.org/10.2144/btn-2019-0156

Author

Farzad, Maryam Sharafi ; Pedersen, Brian Møllegaard ; Mogensen, Helle Smidt ; Børsting, Claus. / Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000. I: BioTechniques. 2020 ; Bind 68, Nr. 6. s. 342-344.

Bibtex

@article{846af829629547388988ec313baa7fce,
title = "Development of an automated AmpliSeq{\texttrademark} library building workflow for biological stain samples on the Biomek{\textregistered} 3000",
abstract = "Here, we present the development of an automated AmpliSeq{\texttrademark} (ThermoFischer, MA, USA) workflow for library building using the Biomek{\textregistered} 3000 Laboratory Automation Workstation (Beckman Coulter Inc., CA, USA), in which the total volume of PCR reagents and reagents for library preparation are reduced by one-half. The automated AmpliSeq workflow was tested using 43 stain samples (blood, bone, muscle tissue, semen, swab, nail scrape and cigarette butts) collected from crime scenes. The sequencing data were evaluated for locus balance, heterozygous allele balance and noise. The performance of libraries built with the automated AmpliSeq workflow using one-half of the recommended reagent volumes were similar to the performance of libraries built with the recommended (full) volumes of the reagents.",
keywords = "AmpliSeq{\texttrademark} workflow, Biomek{\textregistered} 3000, library building, massively parallel sequencing",
author = "Farzad, {Maryam Sharafi} and Pedersen, {Brian M{\o}llegaard} and Mogensen, {Helle Smidt} and Claus B{\o}rsting",
year = "2020",
month = jun,
day = "1",
doi = "10.2144/btn-2019-0156",
language = "English",
volume = "68",
pages = "342--344",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Informa Healthcare",
number = "6",

}

RIS

TY - JOUR

T1 - Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000

AU - Farzad, Maryam Sharafi

AU - Pedersen, Brian Møllegaard

AU - Mogensen, Helle Smidt

AU - Børsting, Claus

PY - 2020/6/1

Y1 - 2020/6/1

N2 - Here, we present the development of an automated AmpliSeq™ (ThermoFischer, MA, USA) workflow for library building using the Biomek® 3000 Laboratory Automation Workstation (Beckman Coulter Inc., CA, USA), in which the total volume of PCR reagents and reagents for library preparation are reduced by one-half. The automated AmpliSeq workflow was tested using 43 stain samples (blood, bone, muscle tissue, semen, swab, nail scrape and cigarette butts) collected from crime scenes. The sequencing data were evaluated for locus balance, heterozygous allele balance and noise. The performance of libraries built with the automated AmpliSeq workflow using one-half of the recommended reagent volumes were similar to the performance of libraries built with the recommended (full) volumes of the reagents.

AB - Here, we present the development of an automated AmpliSeq™ (ThermoFischer, MA, USA) workflow for library building using the Biomek® 3000 Laboratory Automation Workstation (Beckman Coulter Inc., CA, USA), in which the total volume of PCR reagents and reagents for library preparation are reduced by one-half. The automated AmpliSeq workflow was tested using 43 stain samples (blood, bone, muscle tissue, semen, swab, nail scrape and cigarette butts) collected from crime scenes. The sequencing data were evaluated for locus balance, heterozygous allele balance and noise. The performance of libraries built with the automated AmpliSeq workflow using one-half of the recommended reagent volumes were similar to the performance of libraries built with the recommended (full) volumes of the reagents.

KW - AmpliSeq™ workflow

KW - Biomek® 3000

KW - library building

KW - massively parallel sequencing

UR - http://www.scopus.com/inward/record.url?scp=85086525133&partnerID=8YFLogxK

U2 - 10.2144/btn-2019-0156

DO - 10.2144/btn-2019-0156

M3 - Journal article

C2 - 32141765

AN - SCOPUS:85086525133

VL - 68

SP - 342

EP - 344

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 6

ER -

ID: 246786219