CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

CRISPRon/off : CRISPR/Cas9 on- and off-target gRNA design. / Anthon, Christian; Corsi, Giulia Ilaria; Gorodkin, Jan.

I: Bioinformatics, Bind 38, Nr. 24, 2022, s. 5437-5439.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Anthon, C, Corsi, GI & Gorodkin, J 2022, 'CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design', Bioinformatics, bind 38, nr. 24, s. 5437-5439. https://doi.org/10.1093/bioinformatics/btac697

APA

Anthon, C., Corsi, G. I., & Gorodkin, J. (2022). CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design. Bioinformatics, 38(24), 5437-5439. https://doi.org/10.1093/bioinformatics/btac697

Vancouver

Anthon C, Corsi GI, Gorodkin J. CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design. Bioinformatics. 2022;38(24):5437-5439. https://doi.org/10.1093/bioinformatics/btac697

Author

Anthon, Christian ; Corsi, Giulia Ilaria ; Gorodkin, Jan. / CRISPRon/off : CRISPR/Cas9 on- and off-target gRNA design. I: Bioinformatics. 2022 ; Bind 38, Nr. 24. s. 5437-5439.

Bibtex

@article{ac3a58eda48546ffbee101dc9903130e,
title = "CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design",
abstract = "SUMMARY: The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation. Careful design is necessary to select a gRNA with high editing efficiency at the on-target site and with minimum off-target potential. Here, we present our webserver for gRNA design with a user-friendly graphical interface, which provides interoperability between our on- and off-target prediction tools, CRISPRon and CRISPRoff, for a complete and streamlined gRNA selection.AVAILABILITY AND IMPLEMENTATION: The graphical interface uses the Integrative Genomic Viewer (IGV) JavaScript plugin. The backend tools are implemented in Python and C. The CRISPRon and CRISPRoff webservers and command-line tools are freely available at https://rth.dk/resources/crispr.",
keywords = "CRISPR-Cas Systems/genetics, RNA, Guide, CRISPR-Cas Systems, RNA, Guide, Kinetoplastida/genetics, Gene Editing",
author = "Christian Anthon and Corsi, {Giulia Ilaria} and Jan Gorodkin",
note = "{\textcopyright} The Author(s) 2022. Published by Oxford University Press.",
year = "2022",
doi = "10.1093/bioinformatics/btac697",
language = "English",
volume = "38",
pages = "5437--5439",
journal = "Bioinformatics (Online)",
issn = "1367-4811",
publisher = "Oxford University Press",
number = "24",

}

RIS

TY - JOUR

T1 - CRISPRon/off

T2 - CRISPR/Cas9 on- and off-target gRNA design

AU - Anthon, Christian

AU - Corsi, Giulia Ilaria

AU - Gorodkin, Jan

N1 - © The Author(s) 2022. Published by Oxford University Press.

PY - 2022

Y1 - 2022

N2 - SUMMARY: The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation. Careful design is necessary to select a gRNA with high editing efficiency at the on-target site and with minimum off-target potential. Here, we present our webserver for gRNA design with a user-friendly graphical interface, which provides interoperability between our on- and off-target prediction tools, CRISPRon and CRISPRoff, for a complete and streamlined gRNA selection.AVAILABILITY AND IMPLEMENTATION: The graphical interface uses the Integrative Genomic Viewer (IGV) JavaScript plugin. The backend tools are implemented in Python and C. The CRISPRon and CRISPRoff webservers and command-line tools are freely available at https://rth.dk/resources/crispr.

AB - SUMMARY: The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation. Careful design is necessary to select a gRNA with high editing efficiency at the on-target site and with minimum off-target potential. Here, we present our webserver for gRNA design with a user-friendly graphical interface, which provides interoperability between our on- and off-target prediction tools, CRISPRon and CRISPRoff, for a complete and streamlined gRNA selection.AVAILABILITY AND IMPLEMENTATION: The graphical interface uses the Integrative Genomic Viewer (IGV) JavaScript plugin. The backend tools are implemented in Python and C. The CRISPRon and CRISPRoff webservers and command-line tools are freely available at https://rth.dk/resources/crispr.

KW - CRISPR-Cas Systems/genetics

KW - RNA, Guide, CRISPR-Cas Systems

KW - RNA, Guide, Kinetoplastida/genetics

KW - Gene Editing

U2 - 10.1093/bioinformatics/btac697

DO - 10.1093/bioinformatics/btac697

M3 - Journal article

C2 - 36271848

VL - 38

SP - 5437

EP - 5439

JO - Bioinformatics (Online)

JF - Bioinformatics (Online)

SN - 1367-4811

IS - 24

ER -

ID: 339281555