Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent. / Jeppesen, Jacob; Albers, Peter Hjorth; Rose, Adam John; Birk, Jesper Bratz; Schjerling, Peter; Dzamko, Nicolas; Steinberg, Gregory R.; Kiens, Bente.
I: Journal of Lipid Research, Bind 52, Nr. 4, 2011, s. 699-711.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent
AU - Jeppesen, Jacob
AU - Albers, Peter Hjorth
AU - Rose, Adam John
AU - Birk, Jesper Bratz
AU - Schjerling, Peter
AU - Dzamko, Nicolas
AU - Steinberg, Gregory R.
AU - Kiens, Bente
N1 - CURIS 2011 5200 026
PY - 2011
Y1 - 2011
N2 - The aim of this study was to investigate the molecular mechanisms regulating FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. In one model, WT and AMPK KD mice were exercised or EDL and SOL muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and fatty acid uptake in response to muscle contractions was investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, AICAR only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions associated with increased fatty acid uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.
AB - The aim of this study was to investigate the molecular mechanisms regulating FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. In one model, WT and AMPK KD mice were exercised or EDL and SOL muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and fatty acid uptake in response to muscle contractions was investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, AICAR only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions associated with increased fatty acid uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.
U2 - 10.1194/jlr.M007138
DO - 10.1194/jlr.M007138
M3 - Journal article
C2 - 21297178
VL - 52
SP - 699
EP - 711
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 4
ER -
ID: 32928268