Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle

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Standard

Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle. / Alsted, Thomas Junker; Ploug, Thorkil; Prats Gavalda, Clara; Serup, Annette K; Høeg, Louise Dalgas; Schjerling, Peter; Holm, Cecilia; Zimmermann, Robert; Fledelius, Christian; Galbo, Henrik; Kiens, Bente.

I: Journal of Physiology, Bind 591, Nr. 20, 2013, s. 5141-5155.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Alsted, TJ, Ploug, T, Prats Gavalda, C, Serup, AK, Høeg, LD, Schjerling, P, Holm, C, Zimmermann, R, Fledelius, C, Galbo, H & Kiens, B 2013, 'Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle', Journal of Physiology, bind 591, nr. 20, s. 5141-5155. https://doi.org/10.1113/jphysiol.2013.260794

APA

Alsted, T. J., Ploug, T., Prats Gavalda, C., Serup, A. K., Høeg, L. D., Schjerling, P., Holm, C., Zimmermann, R., Fledelius, C., Galbo, H., & Kiens, B. (2013). Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle. Journal of Physiology, 591(20), 5141-5155. https://doi.org/10.1113/jphysiol.2013.260794

Vancouver

Alsted TJ, Ploug T, Prats Gavalda C, Serup AK, Høeg LD, Schjerling P o.a. Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle. Journal of Physiology. 2013;591(20):5141-5155. https://doi.org/10.1113/jphysiol.2013.260794

Author

Alsted, Thomas Junker ; Ploug, Thorkil ; Prats Gavalda, Clara ; Serup, Annette K ; Høeg, Louise Dalgas ; Schjerling, Peter ; Holm, Cecilia ; Zimmermann, Robert ; Fledelius, Christian ; Galbo, Henrik ; Kiens, Bente. / Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle. I: Journal of Physiology. 2013 ; Bind 591, Nr. 20. s. 5141-5155.

Bibtex

@article{2a27febeccca46feadf4edae44047db8,
title = "Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle",
abstract = "In skeletal muscle hormone-sensitive lipase (HSL) has long been accepted to be the principal enzyme responsible for lipolysis of intramyocellular triacylglycerol (IMTG) during contractions. However, this notion is based on in vitro lipase activity data, which may not reflect the in vivo lipolytic activity. We investigated lipolysis of IMTG in soleus muscles electrically-stimulated to contract ex vivo during acute pharmacological inhibition of HSL in rat muscles and in muscles from HSL-KO mice. Measurements of IMTG are complicated by the presence of adipocytes located between the muscle fibers. To circumvent the problem with this contamination we analyzed intramyocellular lipid droplet content histochemically. At maximal inhibition of HSL in rat muscles, contraction-induced breakdown of IMTG was identical to that seen in control muscles (p",
author = "Alsted, {Thomas Junker} and Thorkil Ploug and {Prats Gavalda}, Clara and Serup, {Annette K} and H{\o}eg, {Louise Dalgas} and Peter Schjerling and Cecilia Holm and Robert Zimmermann and Christian Fledelius and Henrik Galbo and Bente Kiens",
note = "CURIS 2013 NEXS 175",
year = "2013",
doi = "10.1113/jphysiol.2013.260794",
language = "English",
volume = "591",
pages = "5141--5155",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "20",

}

RIS

TY - JOUR

T1 - Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle

AU - Alsted, Thomas Junker

AU - Ploug, Thorkil

AU - Prats Gavalda, Clara

AU - Serup, Annette K

AU - Høeg, Louise Dalgas

AU - Schjerling, Peter

AU - Holm, Cecilia

AU - Zimmermann, Robert

AU - Fledelius, Christian

AU - Galbo, Henrik

AU - Kiens, Bente

N1 - CURIS 2013 NEXS 175

PY - 2013

Y1 - 2013

N2 - In skeletal muscle hormone-sensitive lipase (HSL) has long been accepted to be the principal enzyme responsible for lipolysis of intramyocellular triacylglycerol (IMTG) during contractions. However, this notion is based on in vitro lipase activity data, which may not reflect the in vivo lipolytic activity. We investigated lipolysis of IMTG in soleus muscles electrically-stimulated to contract ex vivo during acute pharmacological inhibition of HSL in rat muscles and in muscles from HSL-KO mice. Measurements of IMTG are complicated by the presence of adipocytes located between the muscle fibers. To circumvent the problem with this contamination we analyzed intramyocellular lipid droplet content histochemically. At maximal inhibition of HSL in rat muscles, contraction-induced breakdown of IMTG was identical to that seen in control muscles (p

AB - In skeletal muscle hormone-sensitive lipase (HSL) has long been accepted to be the principal enzyme responsible for lipolysis of intramyocellular triacylglycerol (IMTG) during contractions. However, this notion is based on in vitro lipase activity data, which may not reflect the in vivo lipolytic activity. We investigated lipolysis of IMTG in soleus muscles electrically-stimulated to contract ex vivo during acute pharmacological inhibition of HSL in rat muscles and in muscles from HSL-KO mice. Measurements of IMTG are complicated by the presence of adipocytes located between the muscle fibers. To circumvent the problem with this contamination we analyzed intramyocellular lipid droplet content histochemically. At maximal inhibition of HSL in rat muscles, contraction-induced breakdown of IMTG was identical to that seen in control muscles (p

U2 - 10.1113/jphysiol.2013.260794

DO - 10.1113/jphysiol.2013.260794

M3 - Journal article

C2 - 23878361

VL - 591

SP - 5141

EP - 5155

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 20

ER -

ID: 49037799