Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons
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Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons. / Zhang, Cheng; Svensson, Rene B.; Montagna, Costanza; Carstensen, Helena; Buhl, Rikke; Schoof, Erwin M.; Kjaer, Michael; Magnusson, S. Peter; Yeung, Ching Yan Chloé.
I: Journal of Proteome Research, Bind 19, Nr. 10, 2020, s. 4137-4144.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons
AU - Zhang, Cheng
AU - Svensson, Rene B.
AU - Montagna, Costanza
AU - Carstensen, Helena
AU - Buhl, Rikke
AU - Schoof, Erwin M.
AU - Kjaer, Michael
AU - Magnusson, S. Peter
AU - Yeung, Ching Yan Chloé
PY - 2020
Y1 - 2020
N2 - Tendon is a highly organized, dense connective tissue that has been demonstrated to have very little turnover. In spite of the low turnover, tendon can grow in response to loading, which may take place primarily at the periphery. Tendon injuries and recurrence of injuries are common in both humans and animals in sports. It is unclear why some areas of the tendon are more susceptible to such injuries and whether this is due to intrinsic regional differences in extracellular matrix (ECM) production or tissue turnover. This study aimed to compare populations of tenocytes derived from the tendon core and periphery. Tenocytes were isolated from equine superficial digital flexor tendons (SDFTs), and the proliferation capacity was determined. ECM production was characterized by immuno- and histological staining and by liquid chromatography-mass spectrometry-based proteomics. Core and periphery SDFT cultures exhibited comparable proliferation rates and had very similar proteome profiles, but showed biological variation in collagen type I deposition. In conclusion, the intrinsic properties of tenocytes from different regions of the tendon are very similar, and other factors in the tissue may contribute to how specific areas respond to loading or injury.
AB - Tendon is a highly organized, dense connective tissue that has been demonstrated to have very little turnover. In spite of the low turnover, tendon can grow in response to loading, which may take place primarily at the periphery. Tendon injuries and recurrence of injuries are common in both humans and animals in sports. It is unclear why some areas of the tendon are more susceptible to such injuries and whether this is due to intrinsic regional differences in extracellular matrix (ECM) production or tissue turnover. This study aimed to compare populations of tenocytes derived from the tendon core and periphery. Tenocytes were isolated from equine superficial digital flexor tendons (SDFTs), and the proliferation capacity was determined. ECM production was characterized by immuno- and histological staining and by liquid chromatography-mass spectrometry-based proteomics. Core and periphery SDFT cultures exhibited comparable proliferation rates and had very similar proteome profiles, but showed biological variation in collagen type I deposition. In conclusion, the intrinsic properties of tenocytes from different regions of the tendon are very similar, and other factors in the tissue may contribute to how specific areas respond to loading or injury.
KW - cell proliferation
KW - extracellular matrix
KW - fibroblasts
KW - proteomics
KW - tendon
U2 - 10.1021/acs.jproteome.0c00591
DO - 10.1021/acs.jproteome.0c00591
M3 - Journal article
C2 - 32822197
AN - SCOPUS:85092681596
VL - 19
SP - 4137
EP - 4144
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 10
ER -
ID: 250919682