Combining bleach and mild predigestion improves ancient DNA recovery from bones
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Combining bleach and mild predigestion improves ancient DNA recovery from bones. / Boessenkool, Sanne; Hanghøj, Kristian Ebbesen; Nistelberger, Heidi M.; Der Sarkissian, Clio; Gondek, Agata T.; Orlando, Ludovic Antoine Alexandre; Barrett, James H.; Star, Bastiaan.
I: Molecular Ecology Resources, Bind 17, Nr. 4, 07.2017, s. 742–751.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Combining bleach and mild predigestion improves ancient DNA recovery from bones
AU - Boessenkool, Sanne
AU - Hanghøj, Kristian Ebbesen
AU - Nistelberger, Heidi M.
AU - Der Sarkissian, Clio
AU - Gondek, Agata T.
AU - Orlando, Ludovic Antoine Alexandre
AU - Barrett, James H.
AU - Star, Bastiaan
N1 - © 2016 John Wiley & Sons Ltd.
PY - 2017/7
Y1 - 2017/7
N2 - The feasibility of genome-scale studies from archaeological material remains critically dependent on the ability to access endogenous, authentic DNA. In the majority of cases, this represents a few per cent of the DNA extract, at most. A number of specific pre-extraction protocols for bone powder aimed to improve ancient DNA recovery before library amplification have recently been developed. Here, we test the effects of combining two of such protocols, a bleach wash and a predigestion step, on 12 bone samples of Atlantic cod and domestic horse aged 750-1350 cal. years before present. Using high-throughput sequencing, we show that combined together, bleach wash and predigestion consistently yield DNA libraries with higher endogenous content than either of these methods alone. Additionally, the molecular complexity of these libraries is improved and endogenous DNA templates show larger size distributions. Other library characteristics, such as DNA damage profiles or the composition of microbial communities, are little affected by the pre-extraction protocols. Application of the combined protocol presented in this study will facilitate the genetic analysis of an increasing number of ancient remains and will reduce the cost of whole-genome sequencing.
AB - The feasibility of genome-scale studies from archaeological material remains critically dependent on the ability to access endogenous, authentic DNA. In the majority of cases, this represents a few per cent of the DNA extract, at most. A number of specific pre-extraction protocols for bone powder aimed to improve ancient DNA recovery before library amplification have recently been developed. Here, we test the effects of combining two of such protocols, a bleach wash and a predigestion step, on 12 bone samples of Atlantic cod and domestic horse aged 750-1350 cal. years before present. Using high-throughput sequencing, we show that combined together, bleach wash and predigestion consistently yield DNA libraries with higher endogenous content than either of these methods alone. Additionally, the molecular complexity of these libraries is improved and endogenous DNA templates show larger size distributions. Other library characteristics, such as DNA damage profiles or the composition of microbial communities, are little affected by the pre-extraction protocols. Application of the combined protocol presented in this study will facilitate the genetic analysis of an increasing number of ancient remains and will reduce the cost of whole-genome sequencing.
U2 - 10.1111/1755-0998.12623
DO - 10.1111/1755-0998.12623
M3 - Journal article
C2 - 27790833
VL - 17
SP - 742
EP - 751
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 4
ER -
ID: 172510625