Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection
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Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection. / Sousa-Nunes, Rita; Rana, Amer Ahmed; Kettleborough, Ross; Brickman, Joshua M; Clements, Melanie; Forrest, Alistair; Grimmond, Sean; Avner, Philip; Smith, James C; Dunwoodie, Sally L; Beddington, Rosa S P.
I: Genome Research, Bind 13, Nr. 12, 12.2003, s. 2609-20.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection
AU - Sousa-Nunes, Rita
AU - Rana, Amer Ahmed
AU - Kettleborough, Ross
AU - Brickman, Joshua M
AU - Clements, Melanie
AU - Forrest, Alistair
AU - Grimmond, Sean
AU - Avner, Philip
AU - Smith, James C
AU - Dunwoodie, Sally L
AU - Beddington, Rosa S P
PY - 2003/12
Y1 - 2003/12
N2 - This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.
AB - This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.
KW - Animals
KW - Crosses, Genetic
KW - DNA, Complementary
KW - Endoderm
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Developmental
KW - Gene Library
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Inbred CBA
KW - Sequence Analysis, DNA
U2 - 10.1101/gr.1362303
DO - 10.1101/gr.1362303
M3 - Journal article
C2 - 14613977
VL - 13
SP - 2609
EP - 2620
JO - Genome Research
JF - Genome Research
SN - 1088-9051
IS - 12
ER -
ID: 129061742