TY - JOUR

T1 - Characterization of virus-primed CD8+ T cells with a type 1 cytokine profile

AU - Christensen, Jan Pravsgaard

AU - Stenvang, J P

AU - Marker, O

AU - Thomsen, Allan Randrup

N1 - Keywords: Animals; Cytotoxicity, Immunologic; Female; Hydroxyurea; Inflammation; Integrin alpha4beta1; Integrins; Interferon-gamma; Interleukin-10; Interleukin-5; L-Selectin; Lymphocyte Activation; Lymphocytic Choriomeningitis; Lymphocytic choriomeningitis virus; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Receptors, Lymphocyte Homing; Spleen; T-Lymphocyte Subsets; T-Lymphocytes, Cytotoxic; Tumor Necrosis Factor-alpha

PY - 1996

Y1 - 1996

N2 - Infection with lymphocytic choriomeningitis virus is associated with marked polyclonal activation of the CD8+ T cell subpopulation. In this report the cytokine production of virus-activated T cells is analyzed and the producing cell subset is characterized phenotypically. Coinciding with other parameters of cell-mediated immunity, splenic T cells appear which are able to release high amounts of IFN- gamma, but not IL-5, IL-10 or tumor necrosis factor-alpha upon short-term stimulation with anti-CD3 in vitro. A similar profile is observed analyzing T cells taken from an inflammatory site. Phenotypically, the main cytokine-producing cell subset is found to be CD8+ cells targeted for homing to inflammatory sites (VLA-4hiL-selectinlo) of which 30-40% were positive by intracellular staining for IFN-gamma. This subset also contains all T cells with a cytotoxic potential as measured by redirected killing. An enhanced cytotoxic potential as well as an increased capacity to produce IFN-gamma is observed for at least 2 months after infection and cell sorting analysis revealed that this could be ascribed to a long-standing increase in the frequency of CD8+ Pgp-1hi cells. Therefore, these results demonstrate that systemic virus infection may exert marked perturbation of the CD8+ T cell population resulting in generation of a long-lived subset of primed cells with important effector potential.

AB - Infection with lymphocytic choriomeningitis virus is associated with marked polyclonal activation of the CD8+ T cell subpopulation. In this report the cytokine production of virus-activated T cells is analyzed and the producing cell subset is characterized phenotypically. Coinciding with other parameters of cell-mediated immunity, splenic T cells appear which are able to release high amounts of IFN- gamma, but not IL-5, IL-10 or tumor necrosis factor-alpha upon short-term stimulation with anti-CD3 in vitro. A similar profile is observed analyzing T cells taken from an inflammatory site. Phenotypically, the main cytokine-producing cell subset is found to be CD8+ cells targeted for homing to inflammatory sites (VLA-4hiL-selectinlo) of which 30-40% were positive by intracellular staining for IFN-gamma. This subset also contains all T cells with a cytotoxic potential as measured by redirected killing. An enhanced cytotoxic potential as well as an increased capacity to produce IFN-gamma is observed for at least 2 months after infection and cell sorting analysis revealed that this could be ascribed to a long-standing increase in the frequency of CD8+ Pgp-1hi cells. Therefore, these results demonstrate that systemic virus infection may exert marked perturbation of the CD8+ T cell population resulting in generation of a long-lived subset of primed cells with important effector potential.

M3 - Journal article

C2 - 8921423

VL - 8

SP - 1453

EP - 1461

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 9

ER -