Cellular uptake of metallated cobalamins

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Standard

Cellular uptake of metallated cobalamins. / Tran, Mai Thanh Quynh; Stürup, Stefan; Lambert, Ian Henry; Gammelgaard, Bente; Furger, Evelyne; Alberto, Roger.

I: Metallomics, Bind 8, Nr. 3, 2016, s. 298-304.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Tran, MTQ, Stürup, S, Lambert, IH, Gammelgaard, B, Furger, E & Alberto, R 2016, 'Cellular uptake of metallated cobalamins', Metallomics, bind 8, nr. 3, s. 298-304. https://doi.org/10.1039/c5mt00272a

APA

Tran, M. T. Q., Stürup, S., Lambert, I. H., Gammelgaard, B., Furger, E., & Alberto, R. (2016). Cellular uptake of metallated cobalamins. Metallomics, 8(3), 298-304. https://doi.org/10.1039/c5mt00272a

Vancouver

Tran MTQ, Stürup S, Lambert IH, Gammelgaard B, Furger E, Alberto R. Cellular uptake of metallated cobalamins. Metallomics. 2016;8(3):298-304. https://doi.org/10.1039/c5mt00272a

Author

Tran, Mai Thanh Quynh ; Stürup, Stefan ; Lambert, Ian Henry ; Gammelgaard, Bente ; Furger, Evelyne ; Alberto, Roger. / Cellular uptake of metallated cobalamins. I: Metallomics. 2016 ; Bind 8, Nr. 3. s. 298-304.

Bibtex

@article{cea21e068de14b96872cd15fca814133,
title = "Cellular uptake of metallated cobalamins",
abstract = "Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.",
author = "Tran, {Mai Thanh Quynh} and Stefan St{\"u}rup and Lambert, {Ian Henry} and Bente Gammelgaard and Evelyne Furger and Roger Alberto",
year = "2016",
doi = "10.1039/c5mt00272a",
language = "English",
volume = "8",
pages = "298--304",
journal = "Metallomics",
issn = "1756-5901",
publisher = "Royal Society of Chemistry",
number = "3",

}

RIS

TY - JOUR

T1 - Cellular uptake of metallated cobalamins

AU - Tran, Mai Thanh Quynh

AU - Stürup, Stefan

AU - Lambert, Ian Henry

AU - Gammelgaard, Bente

AU - Furger, Evelyne

AU - Alberto, Roger

PY - 2016

Y1 - 2016

N2 - Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.

AB - Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.

U2 - 10.1039/c5mt00272a

DO - 10.1039/c5mt00272a

M3 - Journal article

C2 - 26739575

VL - 8

SP - 298

EP - 304

JO - Metallomics

JF - Metallomics

SN - 1756-5901

IS - 3

ER -

ID: 156787419