Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.
Originalsprog | Engelsk |
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Tidsskrift | Methods in Molecular Biology |
Vol/bind | 1324 |
Sider (fra-til) | 261-277 |
Antal sider | 17 |
ISSN | 1064-3745 |
DOI | |
Status | Udgivet - 2015 |
Bibliografisk note
Publisher Copyright:
© Springer Science+Business Media New York 2015.
ID: 344326440