TY - JOUR

T1 - Cell transfection as a tool to study growth hormone action

AU - Norstedt, G

AU - Enberg, B

AU - Francis, S

AU - Hansson, A

AU - Hulthén, A

AU - Lobie, P E

AU - Sliva, D

AU - Wood, T J

AU - Billestrup, Nils

PY - 1994/7

Y1 - 1994/7

N2 - The isolation of growth hormone receptor (GHR) cDNA clones has made possible the transfection of GHRs into cultured cells. Our aim in this minireview is to show how the application of such approaches have benefited GHR research. GH stimulation of cells expressing GHR cDNAs can cause an alteration of cellular function that mimic those of the endogenous GHR. GHR cDNA transfected cells also offer a system where the mechanism of GH action can be studied. Such a system has been used to demonstrate that the GHR itself becomes tyrosine phosphorylated and that further phosphorylation of downstream proteins is important in GH action. The GH signals are transmitted to the nucleus and GH regulated genes have now begun to be characterized. The ability to use cell transfection for mechanistic studies of GH action will be instrumental to define domains within the receptor that are of functional importance and to determine pathways whereby GH signals are conveyed within the cell.

AB - The isolation of growth hormone receptor (GHR) cDNA clones has made possible the transfection of GHRs into cultured cells. Our aim in this minireview is to show how the application of such approaches have benefited GHR research. GH stimulation of cells expressing GHR cDNAs can cause an alteration of cellular function that mimic those of the endogenous GHR. GHR cDNA transfected cells also offer a system where the mechanism of GH action can be studied. Such a system has been used to demonstrate that the GHR itself becomes tyrosine phosphorylated and that further phosphorylation of downstream proteins is important in GH action. The GH signals are transmitted to the nucleus and GH regulated genes have now begun to be characterized. The ability to use cell transfection for mechanistic studies of GH action will be instrumental to define domains within the receptor that are of functional importance and to determine pathways whereby GH signals are conveyed within the cell.

KW - Animals

KW - Gene Expression

KW - Growth Hormone

KW - Humans

KW - Receptors, Somatotropin

KW - Signal Transduction

KW - Transfection

M3 - Journal article

C2 - 8016150

VL - 206

SP - 181

EP - 184

JO - Proceedings of the Society for Experimental Biology and Medicine

JF - Proceedings of the Society for Experimental Biology and Medicine

SN - 0037-9727

IS - 3

ER -