C-di-GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa
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Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger which controls the life styles of many bacteria. A high intracellular level of c-di-GMP induces a biofilm lifestyle, whereas a low intracellular level of c-di-GMP stimulates dispersal of biofilms and promotes a planktonic lifestyle. Here, we used expression of different reporters to show that planktonic cells (PCells), biofilm cells (BCells) and cells dispersed from biofilms (DCells) had distinct intracellular c-di-GMP levels. Proteomics analysis showed that the low intracellular c-di-GMP level of DCells induced the expression of proteins required for the virulence and development of antimicrobial peptide resistance in P. aeruginosa. In accordance, P. aeruginosa cells with low c-di-GMP levels were found to be more resistant to colistin than P. aeruginosa cells with high c-di-GMP levels. This contradicts the current dogma stating that dispersed cells are inevitably more susceptible to antibiotics than their sessile counterparts.
Originalsprog | Engelsk |
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Tidsskrift | Antimicrobial Agents and Chemotherapy |
Vol/bind | 57 |
Udgave nummer | 5 |
Sider (fra-til) | 2066-2075 |
Antal sider | 10 |
ISSN | 0066-4804 |
DOI | |
Status | Udgivet - 2013 |
ID: 44310769