Canna edulis leaf extract-mediated preparation of stabilized silver nanoparticles: Characterization, antimicrobial activity, and toxicity studies
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Canna edulis leaf extract-mediated preparation of stabilized silver nanoparticles : Characterization, antimicrobial activity, and toxicity studies. / Otari, S. V.; Pawar, S. H.; Patel, Sanjay K.S.; Singh, Raushan K.; Kim, Sang Yong; Lee, Jai Hyo; Zhang, Liaoyuan; Lee, Jung Kul.
I: Journal of Microbiology and Biotechnology, Bind 27, Nr. 4, 04.2017, s. 731-738.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Canna edulis leaf extract-mediated preparation of stabilized silver nanoparticles
T2 - Characterization, antimicrobial activity, and toxicity studies
AU - Otari, S. V.
AU - Pawar, S. H.
AU - Patel, Sanjay K.S.
AU - Singh, Raushan K.
AU - Kim, Sang Yong
AU - Lee, Jai Hyo
AU - Zhang, Liaoyuan
AU - Lee, Jung Kul
PY - 2017/4
Y1 - 2017/4
N2 - A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract of Canna edulis Ker-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi. The biocompatibility of the AgNPs was analyzed in the L929 cell line using NRU and MTT assays. Acridine orange/ ethidium bromide staining was used to determine whether the AgNPs had necrotic or apoptotic effects on L929 cells. The concentration of AgNPs required for 50% inhibition of growth of mammalian cells is far more than that required for inhibition of pathogenic microorganisms. Thus, CELE is a candidate for the eco-friendly, clean, cost-effective, and nontoxic synthesis of AgNPs.
AB - A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract of Canna edulis Ker-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi. The biocompatibility of the AgNPs was analyzed in the L929 cell line using NRU and MTT assays. Acridine orange/ ethidium bromide staining was used to determine whether the AgNPs had necrotic or apoptotic effects on L929 cells. The concentration of AgNPs required for 50% inhibition of growth of mammalian cells is far more than that required for inhibition of pathogenic microorganisms. Thus, CELE is a candidate for the eco-friendly, clean, cost-effective, and nontoxic synthesis of AgNPs.
KW - Antimicrobial
KW - Biocompatible
KW - Canna edulis Ker-Gawl
KW - Green chemistry
KW - Nanobiotechnology
U2 - 10.4014/jmb.1610.10019
DO - 10.4014/jmb.1610.10019
M3 - Journal article
C2 - 28081356
AN - SCOPUS:85018341752
VL - 27
SP - 731
EP - 738
JO - Microbiology and Biotechnology Letters
JF - Microbiology and Biotechnology Letters
SN - 1598-642X
IS - 4
ER -
ID: 229900768