Butyrate ameliorates allergic airway inflammation by limiting eosinophil trafficking and survival
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Butyrate ameliorates allergic airway inflammation by limiting eosinophil trafficking and survival. / Theiler, Anna; Bärnthaler, Thomas; Platzer, Wolfgang; Richtig, Georg; Peinhaupt, Miriam; Rittchen, Sonja; Kargl, Julia; Ulven, Trond; Marsh, Leigh M; Marsche, Gunther; Schuligoi, Rufina; Sturm, Eva M; Heinemann, Akos.
I: The Journal of allergy and clinical immunology, Bind 144, Nr. 3, 2019, s. 764-776.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Butyrate ameliorates allergic airway inflammation by limiting eosinophil trafficking and survival
AU - Theiler, Anna
AU - Bärnthaler, Thomas
AU - Platzer, Wolfgang
AU - Richtig, Georg
AU - Peinhaupt, Miriam
AU - Rittchen, Sonja
AU - Kargl, Julia
AU - Ulven, Trond
AU - Marsh, Leigh M
AU - Marsche, Gunther
AU - Schuligoi, Rufina
AU - Sturm, Eva M
AU - Heinemann, Akos
N1 - Copyright © 2019. Published by Elsevier Inc.
PY - 2019
Y1 - 2019
N2 - BACKGROUND: Lung eosinophilia is a hallmark of asthma and eosinophils are believed to play a crucial role in the pathogenesis of allergic inflammatory diseases. Short chain fatty acids (SCFA), e.g. acetate, propionate and butyrate are produced in high amounts in the gastro-intestinal tract by commensal bacteria and can be absorbed into the blood stream. Although, there is recent evidence that SCFA are beneficial in allergic asthma models, the effect on eosinophils has remained elusive.OBJECTIVE: The role of SCFA was investigated in human eosinophil function and a mouse model of allergic asthma.METHODS: Eosinophils were purified from self-reported allergic or healthy donors. Migration, adhesion to endothelium and survival of eosinophils was studied in vitro. Ca2+ flux, apoptosis, mitochondrial membrane potential and expression of surface markers was determined by flow cytometry and in part by real time PCR. Allergic airway inflammation was assessed in vivo in an ovalbumin-induced asthma model using invasive spirometry.RESULTS: We observed for the first time that SCFA were able to attenuate human eosinophils at several functional levels including (i) adhesion to the endothelium, (ii) migration and (iii) survival. These effects were independent from GPR41 and GPR43, but were accompanied by histone acetylation and mimicked by TSA, a pan-HDAC inhibitor. In vivo, butyrate ameliorated allergen-induced airway and lung eosinophilia, reduced type 2 cytokines in the bronchial fluid and improved airway hyperresponsiveness in mice.CONCLUSION: These in vitro and in vivo findings highlight the importance of SCFA, especially butyrate as a promising therapeutic agent in allergic inflammatory diseases.
AB - BACKGROUND: Lung eosinophilia is a hallmark of asthma and eosinophils are believed to play a crucial role in the pathogenesis of allergic inflammatory diseases. Short chain fatty acids (SCFA), e.g. acetate, propionate and butyrate are produced in high amounts in the gastro-intestinal tract by commensal bacteria and can be absorbed into the blood stream. Although, there is recent evidence that SCFA are beneficial in allergic asthma models, the effect on eosinophils has remained elusive.OBJECTIVE: The role of SCFA was investigated in human eosinophil function and a mouse model of allergic asthma.METHODS: Eosinophils were purified from self-reported allergic or healthy donors. Migration, adhesion to endothelium and survival of eosinophils was studied in vitro. Ca2+ flux, apoptosis, mitochondrial membrane potential and expression of surface markers was determined by flow cytometry and in part by real time PCR. Allergic airway inflammation was assessed in vivo in an ovalbumin-induced asthma model using invasive spirometry.RESULTS: We observed for the first time that SCFA were able to attenuate human eosinophils at several functional levels including (i) adhesion to the endothelium, (ii) migration and (iii) survival. These effects were independent from GPR41 and GPR43, but were accompanied by histone acetylation and mimicked by TSA, a pan-HDAC inhibitor. In vivo, butyrate ameliorated allergen-induced airway and lung eosinophilia, reduced type 2 cytokines in the bronchial fluid and improved airway hyperresponsiveness in mice.CONCLUSION: These in vitro and in vivo findings highlight the importance of SCFA, especially butyrate as a promising therapeutic agent in allergic inflammatory diseases.
U2 - 10.1016/j.jaci.2019.05.002
DO - 10.1016/j.jaci.2019.05.002
M3 - Journal article
C2 - 31082458
VL - 144
SP - 764
EP - 776
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 3
ER -
ID: 221841283