In recent years, it has become apparent that imbalances in the gastrointestinal system can impact organs beyondthe intestine such as the lungs. Given the established ability of probiotics to modulate the immune system byinteracting with gastrointestinal cells, our research aimed to investigate whether administering the probioticstrain Bacillus subtilis-597 could mitigate the outcome of influenza virus infection in pigs. Pigs were fed a dieteither with or without the probiotic strain B. subtilis-597 for 14 days before being intranasally inoculated with aswine influenza A H1N2 strain (1 C.2 lineage). Throughout the study, we collected fecal samples, blood samples,and nasal swabs to examine viral shedding and immune gene expression. After seven days of infection, the pigswere euthanized, and lung and ileum tissues were collected for gene expression analysis and pathological ex-amination. Our findings indicate that the administration of B. subtilis-597 exhibit potential in reducing lunglesions, possibly attributable to a general suppression of the immune system as indicated by reduced C-reactiveprotein (CRP) levels in serum, decreased expression of interferon-stimulated genes (ISGs), and localized reduc-tion of the inflammatory marker serum amyloid A (SAA) in ileum tissue. Notably, the immune-modulatory effectsof B. subtilis-597 appeared to be unrelated to the gastrointestinal microbiota, as the composition remained un-altered by both the influenza infection and the administration of B. subtilis-597