Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation
Publikation: Bidrag til bog/antologi/rapport › Konferencebidrag i proceedings › Forskning › fagfællebedømt
We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025
Originalsprog | Engelsk |
---|---|
Titel | Progress in Forensic Genetics 13 : Proceedings of the 23rd International ISFG Congress |
Redaktører | Niels Morling |
Antal sider | 2 |
Forlag | Elsevier |
Publikationsdato | 2009 |
Sider | 69-70 |
DOI | |
Status | Udgivet - 2009 |
Begivenhed | 23rd International ISFG Congress - Buenos Aires, Argentina Varighed: 14 sep. 2009 → 18 sep. 2009 |
Konference
Konference | 23rd International ISFG Congress |
---|---|
Land | Argentina |
By | Buenos Aires |
Periode | 14/09/2009 → 18/09/2009 |
Navn | Forensic Science International: Genetics Supplement Series |
---|---|
Nummer | 1 |
Vol/bind | 2 |
ISSN | 1875-1768 |
ID: 14440302