An H+-ATPase assay: Proton pumping and ATPase activity determined simultaneously in the same sample

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Standard

An H+-ATPase assay : Proton pumping and ATPase activity determined simultaneously in the same sample. / Palmgren, Michael Gjedde.

I: Plant Physiology, Bind 94, Nr. 3, 1990, s. 882-886.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Palmgren, MG 1990, 'An H+-ATPase assay: Proton pumping and ATPase activity determined simultaneously in the same sample', Plant Physiology, bind 94, nr. 3, s. 882-886. https://doi.org/10.1104/pp.94.3.882

APA

Palmgren, M. G. (1990). An H+-ATPase assay: Proton pumping and ATPase activity determined simultaneously in the same sample. Plant Physiology, 94(3), 882-886. https://doi.org/10.1104/pp.94.3.882

Vancouver

Palmgren MG. An H+-ATPase assay: Proton pumping and ATPase activity determined simultaneously in the same sample. Plant Physiology. 1990;94(3):882-886. https://doi.org/10.1104/pp.94.3.882

Author

Palmgren, Michael Gjedde. / An H+-ATPase assay : Proton pumping and ATPase activity determined simultaneously in the same sample. I: Plant Physiology. 1990 ; Bind 94, Nr. 3. s. 882-886.

Bibtex

@article{d6327b444c8b467ba4be937fc262b78e,
title = "An H+-ATPase assay: Proton pumping and ATPase activity determined simultaneously in the same sample",
abstract = "A continuous spectrophotometric assay of H+-ATPase activity was developed by combining two well-known methods for measuring proton pumping and ATPase activity. Proton uptake into plasma membrane vesicles from Avena sativa L. (cv Rhiannon) was monitored as the absorbance decrease at 495 nm of the ΔpH probe acridine orange. Simultaneously, ATPase activity was measured by following the absorbance decrease at 340 nanometers by coupling ATP hydrolysis enzymatically to the oxidation of NADH. This H+-ATPase assay is convenient for determining the relative relationship between ATP hydrolysis and proton pumping.",
author = "Palmgren, {Michael Gjedde}",
year = "1990",
doi = "10.1104/pp.94.3.882",
language = "English",
volume = "94",
pages = "882--886",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

RIS

TY - JOUR

T1 - An H+-ATPase assay

T2 - Proton pumping and ATPase activity determined simultaneously in the same sample

AU - Palmgren, Michael Gjedde

PY - 1990

Y1 - 1990

N2 - A continuous spectrophotometric assay of H+-ATPase activity was developed by combining two well-known methods for measuring proton pumping and ATPase activity. Proton uptake into plasma membrane vesicles from Avena sativa L. (cv Rhiannon) was monitored as the absorbance decrease at 495 nm of the ΔpH probe acridine orange. Simultaneously, ATPase activity was measured by following the absorbance decrease at 340 nanometers by coupling ATP hydrolysis enzymatically to the oxidation of NADH. This H+-ATPase assay is convenient for determining the relative relationship between ATP hydrolysis and proton pumping.

AB - A continuous spectrophotometric assay of H+-ATPase activity was developed by combining two well-known methods for measuring proton pumping and ATPase activity. Proton uptake into plasma membrane vesicles from Avena sativa L. (cv Rhiannon) was monitored as the absorbance decrease at 495 nm of the ΔpH probe acridine orange. Simultaneously, ATPase activity was measured by following the absorbance decrease at 340 nanometers by coupling ATP hydrolysis enzymatically to the oxidation of NADH. This H+-ATPase assay is convenient for determining the relative relationship between ATP hydrolysis and proton pumping.

UR - http://www.scopus.com/inward/record.url?scp=0000001959&partnerID=8YFLogxK

U2 - 10.1104/pp.94.3.882

DO - 10.1104/pp.94.3.882

M3 - Journal article

AN - SCOPUS:0000001959

VL - 94

SP - 882

EP - 886

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -

ID: 245001679