Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation

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Standard

Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation. / Sverrild, Asger; Bergqvist, A; Baines, K J; Porsbjerg, C; Andersson, C K; Thomsen, S F; Hoffmann, H.J.; Gibson, P; Erjefält, J S; Backer, Vibeke.

I: Clinical and Experimental Allergy, Bind 46, Nr. 2, 02.2016, s. 288-297.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Sverrild, A, Bergqvist, A, Baines, KJ, Porsbjerg, C, Andersson, CK, Thomsen, SF, Hoffmann, HJ, Gibson, P, Erjefält, JS & Backer, V 2016, 'Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation', Clinical and Experimental Allergy, bind 46, nr. 2, s. 288-297. https://doi.org/10.1111/cea.12609

APA

Sverrild, A., Bergqvist, A., Baines, K. J., Porsbjerg, C., Andersson, C. K., Thomsen, S. F., Hoffmann, H. J., Gibson, P., Erjefält, J. S., & Backer, V. (2016). Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation. Clinical and Experimental Allergy, 46(2), 288-297. https://doi.org/10.1111/cea.12609

Vancouver

Sverrild A, Bergqvist A, Baines KJ, Porsbjerg C, Andersson CK, Thomsen SF o.a. Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation. Clinical and Experimental Allergy. 2016 feb;46(2):288-297. https://doi.org/10.1111/cea.12609

Author

Sverrild, Asger ; Bergqvist, A ; Baines, K J ; Porsbjerg, C ; Andersson, C K ; Thomsen, S F ; Hoffmann, H.J. ; Gibson, P ; Erjefält, J S ; Backer, Vibeke. / Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation. I: Clinical and Experimental Allergy. 2016 ; Bind 46, Nr. 2. s. 288-297.

Bibtex

@article{08c8682eeab441a59589ec94e93b4145,
title = "Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation",
abstract = "BACKGROUND: Airway hyperresponsiveness (AHR) to inhaled mannitol is associated with indirect markers of mast cell activation and eosinophilic airway inflammation. It is unknown how AHR to mannitol relates to mast cell phenotype, mast cell function and measures of eosinophilic inflammation in airway tissue. We compared the number and phenotype of mast cells, mRNA expression of mast cell-associated genes and number of eosinophils in airway tissue of subjects with asthma and healthy controls in relation to AHR to mannitol.METHODS: Airway hyperresponsiveness to inhaled mannitol was measured in 23 non-smoking, corticosteroid-free asthmatic individuals and 10 healthy controls. Mast cells and eosinophils were identified in mucosal biopsies from all participants. Mast cells were divided into phenotypes based on the presence of chymase. mRNA expression of mast cell-associated genes was measured by real-time PCR.RESULTS: The proportion of submucosal MCTC was higher in asthmatic individuals with AHR to mannitol compared with asthmatic individuals without AHR (median: 40.3% vs. 18.7%, P = 0.03). Increased submucosal MCTC numbers were associated with increased levels of mRNA for thymic stromal lymphopoietin (TSLP) and CPA3 in asthmatics. Reactivity to mannitol correlated significantly with eosinophils in submucosa (r(s): 0.56, P = 0.01).CONCLUSION: Airway hyperresponsiveness to inhaled mannitol is associated with an altered submucosal mast cell profile in asthmatic individuals. This mast cell profile is associated with increased levels of TSLP and CPA3. The degree of AHR to mannitol is correlated with the degree of eosinophilic inflammation in the airway submucosa.",
author = "Asger Sverrild and A Bergqvist and Baines, {K J} and C Porsbjerg and Andersson, {C K} and Thomsen, {S F} and H.J. Hoffmann and P Gibson and Erjef{\"a}lt, {J S} and Vibeke Backer",
note = "{\textcopyright} 2015 John Wiley & Sons Ltd.",
year = "2016",
month = feb,
doi = "10.1111/cea.12609",
language = "English",
volume = "46",
pages = "288--297",
journal = "Clinical and Experimental Allergy",
issn = "0954-7894",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Airway responsiveness to mannitol in asthma is associated with chymase-positive mast cells and eosinophilic airway inflammation

AU - Sverrild, Asger

AU - Bergqvist, A

AU - Baines, K J

AU - Porsbjerg, C

AU - Andersson, C K

AU - Thomsen, S F

AU - Hoffmann, H.J.

AU - Gibson, P

AU - Erjefält, J S

AU - Backer, Vibeke

N1 - © 2015 John Wiley & Sons Ltd.

PY - 2016/2

Y1 - 2016/2

N2 - BACKGROUND: Airway hyperresponsiveness (AHR) to inhaled mannitol is associated with indirect markers of mast cell activation and eosinophilic airway inflammation. It is unknown how AHR to mannitol relates to mast cell phenotype, mast cell function and measures of eosinophilic inflammation in airway tissue. We compared the number and phenotype of mast cells, mRNA expression of mast cell-associated genes and number of eosinophils in airway tissue of subjects with asthma and healthy controls in relation to AHR to mannitol.METHODS: Airway hyperresponsiveness to inhaled mannitol was measured in 23 non-smoking, corticosteroid-free asthmatic individuals and 10 healthy controls. Mast cells and eosinophils were identified in mucosal biopsies from all participants. Mast cells were divided into phenotypes based on the presence of chymase. mRNA expression of mast cell-associated genes was measured by real-time PCR.RESULTS: The proportion of submucosal MCTC was higher in asthmatic individuals with AHR to mannitol compared with asthmatic individuals without AHR (median: 40.3% vs. 18.7%, P = 0.03). Increased submucosal MCTC numbers were associated with increased levels of mRNA for thymic stromal lymphopoietin (TSLP) and CPA3 in asthmatics. Reactivity to mannitol correlated significantly with eosinophils in submucosa (r(s): 0.56, P = 0.01).CONCLUSION: Airway hyperresponsiveness to inhaled mannitol is associated with an altered submucosal mast cell profile in asthmatic individuals. This mast cell profile is associated with increased levels of TSLP and CPA3. The degree of AHR to mannitol is correlated with the degree of eosinophilic inflammation in the airway submucosa.

AB - BACKGROUND: Airway hyperresponsiveness (AHR) to inhaled mannitol is associated with indirect markers of mast cell activation and eosinophilic airway inflammation. It is unknown how AHR to mannitol relates to mast cell phenotype, mast cell function and measures of eosinophilic inflammation in airway tissue. We compared the number and phenotype of mast cells, mRNA expression of mast cell-associated genes and number of eosinophils in airway tissue of subjects with asthma and healthy controls in relation to AHR to mannitol.METHODS: Airway hyperresponsiveness to inhaled mannitol was measured in 23 non-smoking, corticosteroid-free asthmatic individuals and 10 healthy controls. Mast cells and eosinophils were identified in mucosal biopsies from all participants. Mast cells were divided into phenotypes based on the presence of chymase. mRNA expression of mast cell-associated genes was measured by real-time PCR.RESULTS: The proportion of submucosal MCTC was higher in asthmatic individuals with AHR to mannitol compared with asthmatic individuals without AHR (median: 40.3% vs. 18.7%, P = 0.03). Increased submucosal MCTC numbers were associated with increased levels of mRNA for thymic stromal lymphopoietin (TSLP) and CPA3 in asthmatics. Reactivity to mannitol correlated significantly with eosinophils in submucosa (r(s): 0.56, P = 0.01).CONCLUSION: Airway hyperresponsiveness to inhaled mannitol is associated with an altered submucosal mast cell profile in asthmatic individuals. This mast cell profile is associated with increased levels of TSLP and CPA3. The degree of AHR to mannitol is correlated with the degree of eosinophilic inflammation in the airway submucosa.

U2 - 10.1111/cea.12609

DO - 10.1111/cea.12609

M3 - Journal article

C2 - 26252943

VL - 46

SP - 288

EP - 297

JO - Clinical and Experimental Allergy

JF - Clinical and Experimental Allergy

SN - 0954-7894

IS - 2

ER -

ID: 162253541