Activation of plant plasma membrane H+-ATPase by the non-ionic detergent Brij 58
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Plasma membrane vesicles were purified from tobacco callii and the modulation of H+-ATPase by detergents was investigated. The nonionic detergent Brij 58 not only activated ATP hydrolysis (2-fold) but also proton pumping (more than 4-fold). Triton X-100, within a more limited concentration range, produced a similar effect. The simultaneous activation of ATP hydrolysis and proton pumping is not compatible with current interpretations of effects of nonionic detergents on the H+-ATPase based on latency of the enzyme and opening of vesicles.
Originalsprog | Engelsk |
---|---|
Tidsskrift | BBA - Biomembranes |
Vol/bind | 1196 |
Udgave nummer | 1 |
Sider (fra-til) | 93-96 |
Antal sider | 4 |
ISSN | 0005-2736 |
DOI | |
Status | Udgivet - 23 nov. 1994 |
ID: 245002558