A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA
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- A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA
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The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL−1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.
Originalsprog | Engelsk |
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Artikelnummer | 5089 |
Tidsskrift | Nature Communications |
Vol/bind | 12 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 2021 |
Bibliografisk note
Funding Information:
This work is financially supported by European Institute of Innovation & Technology (EIT) Health, Project no. 20876.
Publisher Copyright:
© 2021, The Author(s).
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