A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium.
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A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium. / Briand, P.; Petersen, O. W.; Van Deurs, B.
I: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Bind 23, Nr. 3, 03.1987, s. 181-188.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium.
AU - Briand, P.
AU - Petersen, O. W.
AU - Van Deurs, B.
PY - 1987/3
Y1 - 1987/3
N2 - A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone, estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided with apical microvilli. Milk fat globule membrane antigen, specific for the apical membrane domain of normal, luminal breast epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating "stem cell"-like properties. After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell line may represent a useful model for the study of breast cell differentiation and carcinogenesis in vitro.
AB - A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone, estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided with apical microvilli. Milk fat globule membrane antigen, specific for the apical membrane domain of normal, luminal breast epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating "stem cell"-like properties. After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell line may represent a useful model for the study of breast cell differentiation and carcinogenesis in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0023301282&partnerID=8YFLogxK
U2 - 10.1007/BF02623578
DO - 10.1007/BF02623578
M3 - Journal article
C2 - 3558253
AN - SCOPUS:0023301282
VL - 23
SP - 181
EP - 188
JO - In Vitro Cellular & Developmental Biology
JF - In Vitro Cellular & Developmental Biology
SN - 0883-8364
IS - 3
ER -
ID: 347536787