A deeper look at carrier proteome effects for single-cell proteomics
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Multiplexing approaches using tandem mass tags with a carrier proteome to boost sensitivity have advanced single cell proteomics by mass spectrometry (SCoPE-MS). Here, we probe the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. We demonstrate that carrier proteomes, while increasing overall identifications, dictate which proteins are identified. We show that quantitative precision and signal intensity are limited at high carrier levels, hindering the recognition of regulated proteins. Guidelines for optimized mass spectrometry acquisition parameters and best practices for fold-change or protein copy number-based comparisons are provided.
Originalsprog | Engelsk |
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Artikelnummer | 150 |
Tidsskrift | Communications Biology |
Vol/bind | 5 |
Antal sider | 8 |
ISSN | 2399-3642 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
This work was supported by Novo Nordisk Foundation (NNF14CC0001 and NNF17SA0027704), and the program of excellence from the University of Copenhagen (CDO2016). This work has also been supported by EPIC-XS, project number 823839, funded by the Horizon 2020 program of the European Union.
Publisher Copyright:
© 2022, The Author(s).
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