1-Azathioxanthone Appended Lanthanide(III) DO3A Complexes That Luminesce Following Excitation at 405 nm**
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1-Azathioxanthone Appended Lanthanide(III) DO3A Complexes That Luminesce Following Excitation at 405 nm**. / Gundorff Nielsen, Lea; Ravnsborg Hansen, Anne Katrine; Stachelek, Patrycja; Pal, Robert; Just Sørensen, Thomas.
I: European Journal of Inorganic Chemistry, Bind 26, Nr. 24, e202300245, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - 1-Azathioxanthone Appended Lanthanide(III) DO3A Complexes That Luminesce Following Excitation at 405 nm**
AU - Gundorff Nielsen, Lea
AU - Ravnsborg Hansen, Anne Katrine
AU - Stachelek, Patrycja
AU - Pal, Robert
AU - Just Sørensen, Thomas
N1 - Publisher Copyright: © 2023 The Authors. European Journal of Inorganic Chemistry published by Wiley-VCH GmbH.
PY - 2023
Y1 - 2023
N2 - Since the pioneering report by Selvin, we have been fascinated by the potential of using lanthanide luminescence in bioimaging. The uniquely narrow emission lines and long luminescence lifetimes both provide the potential for background free images together with full certainty of probe localization. General use of lanthanide based bioimaging was first challenged by low brightness, and later by the need of UV (<405 nm) excitation sources not present in commercial microscopes. Here, we designed three lanthanide-based imaging probes based on a known motif to investigate the limitations of 405 nm excitation. These were synthesized, characterized, investigated on dedicated as well as commercial microscopes, and the photophysics was explored in detail. It was proven without doubt that the lanthanide complexes enter the cells and luminesce internally. Even so, no lanthanide luminescence were recovered on the commercial microscopes. Thus, we returned to the photophysical properties that afforded the conclusion that – despite the advances in light sources and photodetectors – we need new designs that can give us brighter lanthanide complexes before bioimaging with lanthanide luminescence becomes something that is readily done.
AB - Since the pioneering report by Selvin, we have been fascinated by the potential of using lanthanide luminescence in bioimaging. The uniquely narrow emission lines and long luminescence lifetimes both provide the potential for background free images together with full certainty of probe localization. General use of lanthanide based bioimaging was first challenged by low brightness, and later by the need of UV (<405 nm) excitation sources not present in commercial microscopes. Here, we designed three lanthanide-based imaging probes based on a known motif to investigate the limitations of 405 nm excitation. These were synthesized, characterized, investigated on dedicated as well as commercial microscopes, and the photophysics was explored in detail. It was proven without doubt that the lanthanide complexes enter the cells and luminesce internally. Even so, no lanthanide luminescence were recovered on the commercial microscopes. Thus, we returned to the photophysical properties that afforded the conclusion that – despite the advances in light sources and photodetectors – we need new designs that can give us brighter lanthanide complexes before bioimaging with lanthanide luminescence becomes something that is readily done.
KW - Bioimaging
KW - f-Elements
KW - Lanthanide complexes
KW - Luminescence
KW - Photophysics
U2 - 10.1002/ejic.202300245
DO - 10.1002/ejic.202300245
M3 - Journal article
AN - SCOPUS:85163849098
VL - 26
JO - European Journal of Inorganic Chemistry
JF - European Journal of Inorganic Chemistry
SN - 1434-1948
IS - 24
M1 - e202300245
ER -
ID: 361436366