Cloning and sequencing of the bovine gastrin gene.
Research output: Contribution to journal › Journal article › Research › peer-review
In order to deduce the primary structure of bovine preprogastrin we therefore sequenced a gastrin DNA clone isolated from a bovine liver cosmid library. Bovine preprogastrin comprises 104 amino acids and consists of a signal peptide, a 37 amino acid spacer-sequence, the gastrin-34 sequence followed by an amidation-site (Gly-Arg-Arg), and a C-terminal nonapeptide. Comparison with human, porcine, and rat cDNA sequences revealed extensive homology in the coding region as well as in short noncoding structures.
Original language | English |
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Journal | Molecular Endocrinology |
Volume | 3 |
Issue number | 10 |
Pages (from-to) | 1585-8 |
Number of pages | 3 |
ISSN | 0888-8809 |
Publication status | Published - 1989 |
Bibliographical note
Keywords: Amino Acid Sequence; Animals; Base Sequence; Blotting, Southern; Cattle; Cloning, Molecular; Gastrins; Humans; Liver; Molecular Sequence Data; Rats; Swine
ID: 6586548