Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA

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Standard

Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA. / Lim, Ernest Wee Kiat; Brolin, Camilla; Nielsen, Peter E.

Peptide Nucleic Acids: Methods and Protocols. Humana Press, 2020. s. 251-260 (Methods in Molecular Biology, Bind 2105).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Lim, EWK, Brolin, C & Nielsen, PE 2020, Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA. i Peptide Nucleic Acids: Methods and Protocols. Humana Press, Methods in Molecular Biology, bind 2105, s. 251-260. https://doi.org/10.1007/978-1-0716-0243-0_16

APA

Lim, E. W. K., Brolin, C., & Nielsen, P. E. (2020). Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA. I Peptide Nucleic Acids: Methods and Protocols (s. 251-260). Humana Press. Methods in Molecular Biology Bind 2105 https://doi.org/10.1007/978-1-0716-0243-0_16

Vancouver

Lim EWK, Brolin C, Nielsen PE. Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA. I Peptide Nucleic Acids: Methods and Protocols. Humana Press. 2020. s. 251-260. (Methods in Molecular Biology, Bind 2105). https://doi.org/10.1007/978-1-0716-0243-0_16

Author

Lim, Ernest Wee Kiat ; Brolin, Camilla ; Nielsen, Peter E. / Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA. Peptide Nucleic Acids: Methods and Protocols. Humana Press, 2020. s. 251-260 (Methods in Molecular Biology, Bind 2105).

Bibtex

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title = "Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA",
abstract = "Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.",
keywords = "Biodistribution, IVIS, Tissue accumulation, Whole-body in vivo bioimaging",
author = "Lim, {Ernest Wee Kiat} and Camilla Brolin and Nielsen, {Peter E.}",
year = "2020",
doi = "10.1007/978-1-0716-0243-0_16",
language = "English",
isbn = "978-1-0716-0242-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "251--260",
booktitle = "Peptide Nucleic Acids",
address = "United States",

}

RIS

TY - CHAP

T1 - Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA

AU - Lim, Ernest Wee Kiat

AU - Brolin, Camilla

AU - Nielsen, Peter E.

PY - 2020

Y1 - 2020

N2 - Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.

AB - Using near-infrared fluorophore Alexa Fluor 680 labeled peptide nucleic acids (PNAs) the biodistribution of such antisense agents can be analyzed in real time in live mice using in vivo imaging. Using the fluorescence intensity emitted from the mouse at different time points following administration, the systemic distribution and organ accumulation of PNA can be tracked. In addition, an estimation of the body half-life of the compound can be obtained by the change in fluorescence intensity over time. With this technique, the distribution of compounds can be monitored real time, while reducing the number of animals and amount of compounds required.

KW - Biodistribution

KW - IVIS

KW - Tissue accumulation

KW - Whole-body in vivo bioimaging

U2 - 10.1007/978-1-0716-0243-0_16

DO - 10.1007/978-1-0716-0243-0_16

M3 - Book chapter

C2 - 32088876

AN - SCOPUS:85079912008

SN - 978-1-0716-0242-3

T3 - Methods in Molecular Biology

SP - 251

EP - 260

BT - Peptide Nucleic Acids

PB - Humana Press

ER -

ID: 239812468