MicroRNA Changes in Cerebrospinal Fluid After Subarachnoid Hemorrhage
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MicroRNA Changes in Cerebrospinal Fluid After Subarachnoid Hemorrhage. / Bache, Søren; Rasmussen, Rune; Rossing, Maria; Laigaard, Finn Pedersen; Nielsen, Finn Cilius; Møller, Kirsten.
I: Stroke, Bind 48, Nr. 9, 2017, s. 2391-2398.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - MicroRNA Changes in Cerebrospinal Fluid After Subarachnoid Hemorrhage
AU - Bache, Søren
AU - Rasmussen, Rune
AU - Rossing, Maria
AU - Laigaard, Finn Pedersen
AU - Nielsen, Finn Cilius
AU - Møller, Kirsten
N1 - © 2017 The Authors.
PY - 2017
Y1 - 2017
N2 - BACKGROUND AND PURPOSE: Delayed cerebral ischemia (DCI) accounts for a major part of the morbidity and mortality after aneurysmal subarachnoid hemorrhage (SAH). MicroRNAs (miRNAs) are pathophysiologically involved in acute cerebral ischemia. This study compared miRNA profiles in cerebrospinal fluid from neurologically healthy patients, as well as SAH patients with and without subsequent development of DCI.METHODS: In a prospective case-control study of SAH patients treated with external ventricular drainage and neurologically healthy patients, miRNA profiles in cerebrospinal fluid were screened and validated using 2 different high-throughput real-time quantification polymerase chain reaction techniques. The occurrence of DCI was documented in patient charts and subsequently reviewed independently by 2 physicians.RESULTS: MiRNA profiles from 27 SAH patients and 10 neurologically healthy patients passed quality control. In the validation, 66 miRNAs showed a relative increase in cerebrospinal fluid from SAH patients compared with neurologically healthy patients (P<0.001); 2 (miR-21 and miR-221) showed a relative increase in SAH patients with DCI compared with those without (P<0.05) in both the screening and validation.CONCLUSIONS: SAH is associated with marked changes in the cerebrospinal fluid miRNA profile. These changes could be associated to the development of DCI.CLINICAL TRIAL REGISTRATION: URL: http://www.clinicaltrials.gov. Unique identifier: NCT01791257.
AB - BACKGROUND AND PURPOSE: Delayed cerebral ischemia (DCI) accounts for a major part of the morbidity and mortality after aneurysmal subarachnoid hemorrhage (SAH). MicroRNAs (miRNAs) are pathophysiologically involved in acute cerebral ischemia. This study compared miRNA profiles in cerebrospinal fluid from neurologically healthy patients, as well as SAH patients with and without subsequent development of DCI.METHODS: In a prospective case-control study of SAH patients treated with external ventricular drainage and neurologically healthy patients, miRNA profiles in cerebrospinal fluid were screened and validated using 2 different high-throughput real-time quantification polymerase chain reaction techniques. The occurrence of DCI was documented in patient charts and subsequently reviewed independently by 2 physicians.RESULTS: MiRNA profiles from 27 SAH patients and 10 neurologically healthy patients passed quality control. In the validation, 66 miRNAs showed a relative increase in cerebrospinal fluid from SAH patients compared with neurologically healthy patients (P<0.001); 2 (miR-21 and miR-221) showed a relative increase in SAH patients with DCI compared with those without (P<0.05) in both the screening and validation.CONCLUSIONS: SAH is associated with marked changes in the cerebrospinal fluid miRNA profile. These changes could be associated to the development of DCI.CLINICAL TRIAL REGISTRATION: URL: http://www.clinicaltrials.gov. Unique identifier: NCT01791257.
KW - Adult
KW - Aged
KW - Brain Ischemia/cerebrospinal fluid
KW - Case-Control Studies
KW - Female
KW - High-Throughput Nucleotide Sequencing
KW - Humans
KW - Male
KW - MicroRNAs/cerebrospinal fluid
KW - Middle Aged
KW - Prospective Studies
KW - Real-Time Polymerase Chain Reaction
KW - Sequence Analysis, RNA
KW - Subarachnoid Hemorrhage/cerebrospinal fluid
U2 - 10.1161/STROKEAHA.117.017804
DO - 10.1161/STROKEAHA.117.017804
M3 - Journal article
C2 - 28768799
VL - 48
SP - 2391
EP - 2398
JO - Stroke
JF - Stroke
SN - 0039-2499
IS - 9
ER -
ID: 197003877