TY - JOUR

T1 - MicroRNA Changes in Cerebrospinal Fluid After Subarachnoid Hemorrhage

AU - Bache, Søren

AU - Rasmussen, Rune

AU - Rossing, Maria

AU - Laigaard, Finn Pedersen

AU - Nielsen, Finn Cilius

AU - Møller, Kirsten

N1 - © 2017 The Authors.

PY - 2017

Y1 - 2017

N2 - BACKGROUND AND PURPOSE: Delayed cerebral ischemia (DCI) accounts for a major part of the morbidity and mortality after aneurysmal subarachnoid hemorrhage (SAH). MicroRNAs (miRNAs) are pathophysiologically involved in acute cerebral ischemia. This study compared miRNA profiles in cerebrospinal fluid from neurologically healthy patients, as well as SAH patients with and without subsequent development of DCI.METHODS: In a prospective case-control study of SAH patients treated with external ventricular drainage and neurologically healthy patients, miRNA profiles in cerebrospinal fluid were screened and validated using 2 different high-throughput real-time quantification polymerase chain reaction techniques. The occurrence of DCI was documented in patient charts and subsequently reviewed independently by 2 physicians.RESULTS: MiRNA profiles from 27 SAH patients and 10 neurologically healthy patients passed quality control. In the validation, 66 miRNAs showed a relative increase in cerebrospinal fluid from SAH patients compared with neurologically healthy patients (P<0.001); 2 (miR-21 and miR-221) showed a relative increase in SAH patients with DCI compared with those without (P<0.05) in both the screening and validation.CONCLUSIONS: SAH is associated with marked changes in the cerebrospinal fluid miRNA profile. These changes could be associated to the development of DCI.CLINICAL TRIAL REGISTRATION: URL: http://www.clinicaltrials.gov. Unique identifier: NCT01791257.

AB - BACKGROUND AND PURPOSE: Delayed cerebral ischemia (DCI) accounts for a major part of the morbidity and mortality after aneurysmal subarachnoid hemorrhage (SAH). MicroRNAs (miRNAs) are pathophysiologically involved in acute cerebral ischemia. This study compared miRNA profiles in cerebrospinal fluid from neurologically healthy patients, as well as SAH patients with and without subsequent development of DCI.METHODS: In a prospective case-control study of SAH patients treated with external ventricular drainage and neurologically healthy patients, miRNA profiles in cerebrospinal fluid were screened and validated using 2 different high-throughput real-time quantification polymerase chain reaction techniques. The occurrence of DCI was documented in patient charts and subsequently reviewed independently by 2 physicians.RESULTS: MiRNA profiles from 27 SAH patients and 10 neurologically healthy patients passed quality control. In the validation, 66 miRNAs showed a relative increase in cerebrospinal fluid from SAH patients compared with neurologically healthy patients (P<0.001); 2 (miR-21 and miR-221) showed a relative increase in SAH patients with DCI compared with those without (P<0.05) in both the screening and validation.CONCLUSIONS: SAH is associated with marked changes in the cerebrospinal fluid miRNA profile. These changes could be associated to the development of DCI.CLINICAL TRIAL REGISTRATION: URL: http://www.clinicaltrials.gov. Unique identifier: NCT01791257.

KW - Adult

KW - Aged

KW - Brain Ischemia/cerebrospinal fluid

KW - Case-Control Studies

KW - Female

KW - High-Throughput Nucleotide Sequencing

KW - Humans

KW - Male

KW - MicroRNAs/cerebrospinal fluid

KW - Middle Aged

KW - Prospective Studies

KW - Real-Time Polymerase Chain Reaction

KW - Sequence Analysis, RNA

KW - Subarachnoid Hemorrhage/cerebrospinal fluid

U2 - 10.1161/STROKEAHA.117.017804

DO - 10.1161/STROKEAHA.117.017804

M3 - Journal article

C2 - 28768799

VL - 48

SP - 2391

EP - 2398

JO - Stroke

JF - Stroke

SN - 0039-2499

IS - 9

ER -