In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning
CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver "naked" plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.
Originalsprog | Engelsk |
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Titel | CRISPR Gene Editing : Methods and Protocols |
Redaktører | Yonglun Luo |
Antal sider | 13 |
Vol/bind | 1961 |
Udgivelsessted | New York, NY |
Forlag | Humana Press |
Publikationsdato | 2019 |
Sider | 329-341 |
Artikelnummer | 20 |
Kapitel | 5 |
ISBN (Trykt) | 978-1-4939-9169-3 |
ISBN (Elektronisk) | 978-1-4939-9170-9 |
DOI | |
Status | Udgivet - 2019 |
Navn | Methods in molecular biology (Clifton, N.J.) |
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ISSN | 1064-3745 |
ID: 231415213