Development of a fiber-based microfluidic flow cytometry platform using viscoelastic fluids for polydisperse particle suspensions
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Flow cytometry (FC) is a pivotal tool for studying the physical and chemical properties of particles. State-of-the-art FC systems are highly advanced, yet they are expensive, bulky, and require high sample volume, qualified operators, and periodic maintenance. The manipulation of particles suspended in viscoelastic fluids has received increasing attention, especially for miniaturized flow cytometry technologies. This study presents a miniaturized optical capillary FC device using the viscoelastic focusing technique. A straight, one inlet/outlet microcapillary device is precisely aligned to a fiber-coupled laser source and detectors. Forward scattered, side scattered, and fluorescently emitted light signals are collected and analyzed in a real-time environment. The developed platform fits onto an inverted microscope stage enabling real-time microscopy imaging of the particles of interest together with the flow cytometry analysis. We achieved stable viscoelastic focusing and performed FC measurements for rigid polystyrene beads (diameters: 2 - 15 μm), non-spherical human erythrocytes, and canonical shape metaphase human chromosomes. We performed cytometry measurements with a throughput of 100 events/s yielding a coefficient of variation of 2%. This newly developed FC device is a versatile tool and can be operated with any inverted microscope to get the mutual benefits of optical and imaging FC measurements. Furthermore, it is possible to extend these benefits by adding more back-end tools, such as optical trapping and Raman spectroscopy.
Originalsprog | Engelsk |
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Artikelnummer | 1219802 |
Tidsskrift | Proceedings of SPIE - The International Society for Optical Engineering |
Vol/bind | 12198 |
Antal sider | 9 |
ISSN | 0277-786X |
DOI | |
Status | Udgivet - 2022 |
Begivenhed | Optical Trapping and Optical Micromanipulation XIX 2022 - San Diego, USA Varighed: 21 aug. 2022 → 24 aug. 2022 |
Konference
Konference | Optical Trapping and Optical Micromanipulation XIX 2022 |
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Land | USA |
By | San Diego |
Periode | 21/08/2022 → 24/08/2022 |
Sponsor | The Society of Photo-Optical Instrumentation Engineers (SPIE) |
Bibliografisk note
Funding Information:
This work was partly funded by NovoNordisk Foundation Interdisciplinary Synergy Programme, project ChromoCapture (grant number: NNF18OC0034948). CFN and IDH are supported by the Danish National Research Foundation (grant number: DNRF115). MN and KBS are supported by the Independent Research Fund Denmark (grant no 0135-00142B) and the Novo Nordisk Foundation (grant number: NNF20OC0061673). MN and KBS acknowledge fruitful discussions with Martin Dufva. MN and MS acknowledge Bente Rotbøl for technical support on flow cytometry. MS acknowledges Per Thor Jonassen and Henning Engelbrecht Larsen for their technical support.
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Copyright © 2022 SPIE.
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