CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design
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SUMMARY: The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation. Careful design is necessary to select a gRNA with high editing efficiency at the on-target site and with minimum off-target potential. Here, we present our webserver for gRNA design with a user-friendly graphical interface, which provides interoperability between our on- and off-target prediction tools, CRISPRon and CRISPRoff, for a complete and streamlined gRNA selection.
AVAILABILITY AND IMPLEMENTATION: The graphical interface uses the Integrative Genomic Viewer (IGV) JavaScript plugin. The backend tools are implemented in Python and C. The CRISPRon and CRISPRoff webservers and command-line tools are freely available at https://rth.dk/resources/crispr.
Originalsprog | Engelsk |
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Tidsskrift | Bioinformatics |
Vol/bind | 38 |
Udgave nummer | 24 |
Sider (fra-til) | 5437-5439 |
Antal sider | 3 |
ISSN | 1367-4811 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
© The Author(s) 2022. Published by Oxford University Press.
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