Chromosome-scale assembly and whole-genome sequencing of 266 giant panda roundworms provide insights into their evolution, adaptation and potential drug targets
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Chromosome-scale assembly and whole-genome sequencing of 266 giant panda roundworms provide insights into their evolution, adaptation and potential drug targets. / Han, Lei; Lan, Tianming; Li, Desheng; Li, Haimeng; Deng, Linhua; Peng, Zhiwei; He, Shaowen; Zhou, Yanqiang; Han, Ruobing; Li, Lingling; Lu, Yaxian; Lu, Haorong; Wang, Qing; Yang, Shangchen; Zhu, Yixin; Huang, Yunting; Cheng, Xiaofang; Yu, Jieyao; Wang, Yulong; Sun, Heting; Chai, Hongliang; Yang, Huanming; Xu, Xun; Lisby, Michael; Liu, Quan; Kristiansen, Karsten; Liu, Huan; Hou, Zhijun.
I: Molecular Ecology Resources, Bind 22, Nr. 2, 2022, s. 768-785.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Chromosome-scale assembly and whole-genome sequencing of 266 giant panda roundworms provide insights into their evolution, adaptation and potential drug targets
AU - Han, Lei
AU - Lan, Tianming
AU - Li, Desheng
AU - Li, Haimeng
AU - Deng, Linhua
AU - Peng, Zhiwei
AU - He, Shaowen
AU - Zhou, Yanqiang
AU - Han, Ruobing
AU - Li, Lingling
AU - Lu, Yaxian
AU - Lu, Haorong
AU - Wang, Qing
AU - Yang, Shangchen
AU - Zhu, Yixin
AU - Huang, Yunting
AU - Cheng, Xiaofang
AU - Yu, Jieyao
AU - Wang, Yulong
AU - Sun, Heting
AU - Chai, Hongliang
AU - Yang, Huanming
AU - Xu, Xun
AU - Lisby, Michael
AU - Liu, Quan
AU - Kristiansen, Karsten
AU - Liu, Huan
AU - Hou, Zhijun
N1 - Publisher Copyright: © 2021 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
PY - 2022
Y1 - 2022
N2 - Helminth diseases have long been a threat to the health of humans and animals. Roundworms are important organisms for studying parasitic mechanisms, disease transmission and prevention. The study of parasites in the giant panda is of importance for understanding how roundworms adapt to the host. Here, we report a high-quality chromosome-scale genome of Baylisascaris schroederi with a genome size of 253.60 Mb and 19,262 predicted protein-coding genes. We found that gene families related to epidermal chitin synthesis and environmental information processes in the roundworm genome have expanded significantly. Furthermore, we demonstrated unique genes involved in essential amino acid metabolism in the B. schroederi genome, inferred to be essential for the adaptation to the giant panda-specific diet. In addition, under different deworming pressures, we found that four resistance-related genes (glc-1, nrf-6, bre-4 and ced-7) were under strong positive selection in a captive population. Finally, 23 known drug targets and 47 potential drug target proteins were identified. The genome provides a unique reference for inferring the early evolution of roundworms and their adaptation to the host. Population genetic analysis and drug sensitivity prediction provide insights revealing the impact of deworming history on population genetic structure of importance for disease prevention.
AB - Helminth diseases have long been a threat to the health of humans and animals. Roundworms are important organisms for studying parasitic mechanisms, disease transmission and prevention. The study of parasites in the giant panda is of importance for understanding how roundworms adapt to the host. Here, we report a high-quality chromosome-scale genome of Baylisascaris schroederi with a genome size of 253.60 Mb and 19,262 predicted protein-coding genes. We found that gene families related to epidermal chitin synthesis and environmental information processes in the roundworm genome have expanded significantly. Furthermore, we demonstrated unique genes involved in essential amino acid metabolism in the B. schroederi genome, inferred to be essential for the adaptation to the giant panda-specific diet. In addition, under different deworming pressures, we found that four resistance-related genes (glc-1, nrf-6, bre-4 and ced-7) were under strong positive selection in a captive population. Finally, 23 known drug targets and 47 potential drug target proteins were identified. The genome provides a unique reference for inferring the early evolution of roundworms and their adaptation to the host. Population genetic analysis and drug sensitivity prediction provide insights revealing the impact of deworming history on population genetic structure of importance for disease prevention.
KW - adaptation
KW - anthelmintics
KW - Baylisascaris schroederi
KW - genetic diversity
KW - roundworms
U2 - 10.1111/1755-0998.13504
DO - 10.1111/1755-0998.13504
M3 - Journal article
C2 - 34549895
AN - SCOPUS:85118228843
VL - 22
SP - 768
EP - 785
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 2
ER -
ID: 285241070