Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level.
Originalsprog | Engelsk |
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Titel | P-Type ATPases : methods and protocols |
Redaktører | Maike Bublitz |
Antal sider | 11 |
Vol/bind | 2. |
Forlag | Springer |
Publikationsdato | 2016 |
Sider | 181-191 |
Kapitel | 18 |
ISBN (Trykt) | 978-1-4939-3178-1 |
ISBN (Elektronisk) | 978-1-4939-3179-8 |
DOI | |
Status | Udgivet - 2016 |
Navn | Methods in Molecular Biology |
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Vol/bind | 1377 |
ISSN | 1064-3745 |
ID: 169109211