Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. / Bernabe, Cristian S.; Caliman, Izabela F.; Truitt, William A.; Molosh, Andrei I.; Lowry, Christopher A.; Hay-Schmidt, Anders; Shekhar, Anantha; Johnson, Philip L.

In: Journal of psychopharmacology (Oxford, England), Vol. 34, No. 4, 2020, p. 400-411.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bernabe, CS, Caliman, IF, Truitt, WA, Molosh, AI, Lowry, CA, Hay-Schmidt, A, Shekhar, A & Johnson, PL 2020, 'Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors', Journal of psychopharmacology (Oxford, England), vol. 34, no. 4, pp. 400-411. https://doi.org/10.1177/0269881119900981

APA

Bernabe, C. S., Caliman, I. F., Truitt, W. A., Molosh, A. I., Lowry, C. A., Hay-Schmidt, A., Shekhar, A., & Johnson, P. L. (2020). Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. Journal of psychopharmacology (Oxford, England), 34(4), 400-411. https://doi.org/10.1177/0269881119900981

Vancouver

Bernabe CS, Caliman IF, Truitt WA, Molosh AI, Lowry CA, Hay-Schmidt A et al. Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. Journal of psychopharmacology (Oxford, England). 2020;34(4):400-411. https://doi.org/10.1177/0269881119900981

Author

Bernabe, Cristian S. ; Caliman, Izabela F. ; Truitt, William A. ; Molosh, Andrei I. ; Lowry, Christopher A. ; Hay-Schmidt, Anders ; Shekhar, Anantha ; Johnson, Philip L. / Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. In: Journal of psychopharmacology (Oxford, England). 2020 ; Vol. 34, No. 4. pp. 400-411.

Bibtex

@article{e77b2fe3974c41ee835eac0ebe6a6fee,
title = "Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors",
abstract = "BACKGROUND: The central serotonergic system originating from the dorsal raphe nucleus (DR) plays a critical role in anxiety and trauma-related disorders such as posttraumatic stress disorder. Although many studies have investigated the role of serotonin (5-HT) within pro-fear brain regions such as the amygdala, the majority of these studies have utilized non-selective pharmacological approaches or poorly understood lesioning techniques which limit their interpretation.AIM: Here we investigated the role of amygdala-projecting 5-HT neurons in the DR in innate anxiety and conditioned fear behaviors.METHODS: To achieve this goal, we utilized (1) selective lesion of 5-HT neurons projecting to the amygdala with saporin toxin conjugated to anti-serotonin transporter (SERT) injected into the amygdala, and (2) optogenetic excitation of amygdala-projecting DR cell bodies with a combination of a retrogradely transported canine adenovirus-expressing Cre-recombinase injected into the amygdala and a Cre-dependent-channelrhodopsin injected into the DR.RESULTS: While saporin treatment lesioned both local amygdalar 5-HT fibers and neurons in the DR as well as reduced conditioned fear behavior, optical activation of amygdala-projecting DR neurons enhanced anxious behavior and conditioned fear response.CONCLUSION: Collectively, these studies support the hypothesis that amygdala-projecting 5-HT neurons in the DR represent an anxiety and fear-on network.",
author = "Bernabe, {Cristian S.} and Caliman, {Izabela F.} and Truitt, {William A.} and Molosh, {Andrei I.} and Lowry, {Christopher A.} and Anders Hay-Schmidt and Anantha Shekhar and Johnson, {Philip L.}",
year = "2020",
doi = "10.1177/0269881119900981",
language = "English",
volume = "34",
pages = "400--411",
journal = "Journal of Psychopharmacology",
issn = "0269-8811",
publisher = "SAGE Publications",
number = "4",

}

RIS

TY - JOUR

T1 - Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors

AU - Bernabe, Cristian S.

AU - Caliman, Izabela F.

AU - Truitt, William A.

AU - Molosh, Andrei I.

AU - Lowry, Christopher A.

AU - Hay-Schmidt, Anders

AU - Shekhar, Anantha

AU - Johnson, Philip L.

PY - 2020

Y1 - 2020

N2 - BACKGROUND: The central serotonergic system originating from the dorsal raphe nucleus (DR) plays a critical role in anxiety and trauma-related disorders such as posttraumatic stress disorder. Although many studies have investigated the role of serotonin (5-HT) within pro-fear brain regions such as the amygdala, the majority of these studies have utilized non-selective pharmacological approaches or poorly understood lesioning techniques which limit their interpretation.AIM: Here we investigated the role of amygdala-projecting 5-HT neurons in the DR in innate anxiety and conditioned fear behaviors.METHODS: To achieve this goal, we utilized (1) selective lesion of 5-HT neurons projecting to the amygdala with saporin toxin conjugated to anti-serotonin transporter (SERT) injected into the amygdala, and (2) optogenetic excitation of amygdala-projecting DR cell bodies with a combination of a retrogradely transported canine adenovirus-expressing Cre-recombinase injected into the amygdala and a Cre-dependent-channelrhodopsin injected into the DR.RESULTS: While saporin treatment lesioned both local amygdalar 5-HT fibers and neurons in the DR as well as reduced conditioned fear behavior, optical activation of amygdala-projecting DR neurons enhanced anxious behavior and conditioned fear response.CONCLUSION: Collectively, these studies support the hypothesis that amygdala-projecting 5-HT neurons in the DR represent an anxiety and fear-on network.

AB - BACKGROUND: The central serotonergic system originating from the dorsal raphe nucleus (DR) plays a critical role in anxiety and trauma-related disorders such as posttraumatic stress disorder. Although many studies have investigated the role of serotonin (5-HT) within pro-fear brain regions such as the amygdala, the majority of these studies have utilized non-selective pharmacological approaches or poorly understood lesioning techniques which limit their interpretation.AIM: Here we investigated the role of amygdala-projecting 5-HT neurons in the DR in innate anxiety and conditioned fear behaviors.METHODS: To achieve this goal, we utilized (1) selective lesion of 5-HT neurons projecting to the amygdala with saporin toxin conjugated to anti-serotonin transporter (SERT) injected into the amygdala, and (2) optogenetic excitation of amygdala-projecting DR cell bodies with a combination of a retrogradely transported canine adenovirus-expressing Cre-recombinase injected into the amygdala and a Cre-dependent-channelrhodopsin injected into the DR.RESULTS: While saporin treatment lesioned both local amygdalar 5-HT fibers and neurons in the DR as well as reduced conditioned fear behavior, optical activation of amygdala-projecting DR neurons enhanced anxious behavior and conditioned fear response.CONCLUSION: Collectively, these studies support the hypothesis that amygdala-projecting 5-HT neurons in the DR represent an anxiety and fear-on network.

U2 - 10.1177/0269881119900981

DO - 10.1177/0269881119900981

M3 - Journal article

C2 - 32153226

VL - 34

SP - 400

EP - 411

JO - Journal of Psychopharmacology

JF - Journal of Psychopharmacology

SN - 0269-8811

IS - 4

ER -

ID: 237515851