Typing of 49 autosomal SNPs by single base extension and capillary electrophoresis for forensic genetic testing
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Typing of 49 autosomal SNPs by single base extension and capillary electrophoresis for forensic genetic testing. / Børsting, Claus; Tomas Mas, Carmen; Morling, Niels.
In: Methods in molecular biology (Clifton, N.J.), Vol. 830, 2012, p. 87-107.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Typing of 49 autosomal SNPs by single base extension and capillary electrophoresis for forensic genetic testing
AU - Børsting, Claus
AU - Tomas Mas, Carmen
AU - Morling, Niels
PY - 2012
Y1 - 2012
N2 - We describe a method for simultaneous amplification of 49 autosomal single nucleotide polymorphisms (SNPs) by multiplex PCR and detection of the SNP alleles by single base extension (SBE) and capillary electrophoresis. All the SNPs may be amplified from only 100 pg of genomic DNA and the length of the amplicons range from 65 to 115 bp. The high sensitivity and the short amplicon sizes make the assay very suitable for typing of degraded DNA samples, and the low mutation rate of SNPs makes the assay very useful for relationship testing. Combined, these advantages make the assay well suited for disaster victim identifications, where the DNA from the victims may be highly degraded and the victims are identified via investigation of their relatives. The assay was validated according to the ISO 17025 standard and used for routine case work in our laboratory.
AB - We describe a method for simultaneous amplification of 49 autosomal single nucleotide polymorphisms (SNPs) by multiplex PCR and detection of the SNP alleles by single base extension (SBE) and capillary electrophoresis. All the SNPs may be amplified from only 100 pg of genomic DNA and the length of the amplicons range from 65 to 115 bp. The high sensitivity and the short amplicon sizes make the assay very suitable for typing of degraded DNA samples, and the low mutation rate of SNPs makes the assay very useful for relationship testing. Combined, these advantages make the assay well suited for disaster victim identifications, where the DNA from the victims may be highly degraded and the victims are identified via investigation of their relatives. The assay was validated according to the ISO 17025 standard and used for routine case work in our laboratory.
KW - Base Pairing
KW - Chromosomes, Human
KW - DNA
KW - DNA Primers
KW - Electrophoresis, Capillary
KW - Forensic Genetics
KW - Genotyping Techniques
KW - Humans
KW - Oligonucleotides
KW - Polymerase Chain Reaction
KW - Polymorphism, Single Nucleotide
U2 - 10.1007/978-1-61779-461-2_7
DO - 10.1007/978-1-61779-461-2_7
M3 - Journal article
C2 - 22139655
VL - 830
SP - 87
EP - 107
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -
ID: 38292987