Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.
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Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1. / Gerdes, K; Helin, K; Christensen, O W; Løbner-Olesen, A.
In: Journal of Molecular Biology, Vol. 203, No. 1, 1988, p. 119-29.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Translational control and differential RNA decay are key elements regulating postsegregational expression of the killer protein encoded by the parB locus of plasmid R1.
AU - Gerdes, K
AU - Helin, K
AU - Christensen, O W
AU - Løbner-Olesen, A
N1 - Keywords: Base Sequence; Chromosome Mapping; Escherichia coli; Gene Expression Regulation; Genes, Bacterial; Molecular Sequence Data; Promoter Regions (Genetics); Protein Biosynthesis; R Factors; RNA, Bacterial; RNA, Messenger; Rifampin; Suppression, Genetic
PY - 1988
Y1 - 1988
N2 - The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA complementary to the hok mRNA. The hok mRNA is extraordinarily stable, while the sok RNA decays rapidly. The mechanism of postsegregational killing is explained by the following model; the sok RNA molecule rapidly disappears in cells that have lost a parB-carrying plasmid, leading to translation of the stable hok mRNA. Consequently, the Hok protein is synthesized and killing of the plasmid-free cell follows.
AB - The parB locus of plasmid R1, which mediates plasmid stability via postsegregational killing of plasmid-free cells, encodes two genes, hok and sok. The hok gene product is a potent cell-killing protein. The hok gene is regulated at the translational level by the sok gene-encoded repressor, a small anti-sense RNA complementary to the hok mRNA. The hok mRNA is extraordinarily stable, while the sok RNA decays rapidly. The mechanism of postsegregational killing is explained by the following model; the sok RNA molecule rapidly disappears in cells that have lost a parB-carrying plasmid, leading to translation of the stable hok mRNA. Consequently, the Hok protein is synthesized and killing of the plasmid-free cell follows.
M3 - Journal article
C2 - 2460630
VL - 203
SP - 119
EP - 129
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -
ID: 5053487