Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation

Research output: Contribution to journal › Journal article › Research › peer-review

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Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation. / Redó-Riveiro, Alba; Al-Mousawi, Jasmina; Linneberg-Agerholm, Madeleine; Proks, Martin; Perera, Marta; Salehin, Nazmus; Brickman, Joshua M.

In: Stem Cell Reports, Vol. 19, No. 2, 2024, p. 174-186.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Redó-Riveiro, A, Al-Mousawi, J, Linneberg-Agerholm, M, Proks, M, Perera, M, Salehin, N & Brickman, JM 2024, 'Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation', Stem Cell Reports, vol. 19, no. 2, pp. 174-186. https://doi.org/10.1016/j.stemcr.2023.12.002

APA

Redó-Riveiro, A., Al-Mousawi, J., Linneberg-Agerholm, M., Proks, M., Perera, M., Salehin, N., & Brickman, J. M. (2024). Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation. Stem Cell Reports, 19(2), 174-186. https://doi.org/10.1016/j.stemcr.2023.12.002

Vancouver

Redó-Riveiro A, Al-Mousawi J, Linneberg-Agerholm M, Proks M, Perera M, Salehin N et al. Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation. Stem Cell Reports. 2024;19(2):174-186. https://doi.org/10.1016/j.stemcr.2023.12.002

Author

Redó-Riveiro, Alba ; Al-Mousawi, Jasmina ; Linneberg-Agerholm, Madeleine ; Proks, Martin ; Perera, Marta ; Salehin, Nazmus ; Brickman, Joshua M. / Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation. In: Stem Cell Reports. 2024 ; Vol. 19, No. 2. pp. 174-186.

Bibtex

@article{e1ec4898aa0f4b338787d317e5f9afb1,
title = "Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation",
abstract = "In early mammalian development, cleavage stage blastomeres and inner cell mass (ICM) cells co-express embryonic and extra-embryonic transcriptional determinants. Using a protein-based double reporter we identify an embryonic stem cell (ESC) population that co-expresses the extra-embryonic factor GATA6 alongside the embryonic factor SOX2. Based on single cell transcriptomics, we find this population resembles the unsegregated ICM, exhibiting enhanced differentiation potential for endoderm while maintaining epiblast competence. To relate transcription factor binding in these cells to future fate, we describe a complete enhancer set in both ESCs and naive extra-embryonic endoderm stem cells and assess SOX2 and GATA6 binding at these elements in the ICM-like ESC sub-population. Both factors support cooperative recognition in these lineages, with GATA6 bound alongside SOX2 on a fraction of pluripotency enhancers and SOX2 alongside GATA6 more extensively on endoderm enhancers, suggesting that cooperative binding between these antagonistic factors both supports self-renewal and prepares progenitor cells for later differentiation.",
keywords = "Blastocyst, Cooperativity, Embryonic Stem Cells, Endoderm, Enhancer, Epiblast, Lineage Priming, nEnd, Pluripotency, Transcription",
author = "Alba Red{\'o}-Riveiro and Jasmina Al-Mousawi and Madeleine Linneberg-Agerholm and Martin Proks and Marta Perera and Nazmus Salehin and Brickman, {Joshua M.}",
note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2024",
doi = "10.1016/j.stemcr.2023.12.002",
language = "English",
volume = "19",
pages = "174--186",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation

AU - Redó-Riveiro, Alba

AU - Al-Mousawi, Jasmina

AU - Linneberg-Agerholm, Madeleine

AU - Proks, Martin

AU - Perera, Marta

AU - Salehin, Nazmus

AU - Brickman, Joshua M.

N1 - Publisher Copyright: © 2023 The Author(s)

PY - 2024

Y1 - 2024

N2 - In early mammalian development, cleavage stage blastomeres and inner cell mass (ICM) cells co-express embryonic and extra-embryonic transcriptional determinants. Using a protein-based double reporter we identify an embryonic stem cell (ESC) population that co-expresses the extra-embryonic factor GATA6 alongside the embryonic factor SOX2. Based on single cell transcriptomics, we find this population resembles the unsegregated ICM, exhibiting enhanced differentiation potential for endoderm while maintaining epiblast competence. To relate transcription factor binding in these cells to future fate, we describe a complete enhancer set in both ESCs and naive extra-embryonic endoderm stem cells and assess SOX2 and GATA6 binding at these elements in the ICM-like ESC sub-population. Both factors support cooperative recognition in these lineages, with GATA6 bound alongside SOX2 on a fraction of pluripotency enhancers and SOX2 alongside GATA6 more extensively on endoderm enhancers, suggesting that cooperative binding between these antagonistic factors both supports self-renewal and prepares progenitor cells for later differentiation.

AB - In early mammalian development, cleavage stage blastomeres and inner cell mass (ICM) cells co-express embryonic and extra-embryonic transcriptional determinants. Using a protein-based double reporter we identify an embryonic stem cell (ESC) population that co-expresses the extra-embryonic factor GATA6 alongside the embryonic factor SOX2. Based on single cell transcriptomics, we find this population resembles the unsegregated ICM, exhibiting enhanced differentiation potential for endoderm while maintaining epiblast competence. To relate transcription factor binding in these cells to future fate, we describe a complete enhancer set in both ESCs and naive extra-embryonic endoderm stem cells and assess SOX2 and GATA6 binding at these elements in the ICM-like ESC sub-population. Both factors support cooperative recognition in these lineages, with GATA6 bound alongside SOX2 on a fraction of pluripotency enhancers and SOX2 alongside GATA6 more extensively on endoderm enhancers, suggesting that cooperative binding between these antagonistic factors both supports self-renewal and prepares progenitor cells for later differentiation.

KW - Blastocyst

KW - Cooperativity

KW - Embryonic Stem Cells

KW - Endoderm

KW - Enhancer

KW - Epiblast

KW - Lineage Priming

KW - nEnd

KW - Pluripotency

KW - Transcription

U2 - 10.1016/j.stemcr.2023.12.002

DO - 10.1016/j.stemcr.2023.12.002

M3 - Journal article

C2 - 38215757

AN - SCOPUS:85184607262

VL - 19

SP - 174

EP - 186

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 2

ER -

ID: 382985717