Tobacco smoking and oxidative stress to DNA: a meta-analysis of studies using chromatographic and immunological methods

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Tobacco smoking and oxidative stress to DNA : a meta-analysis of studies using chromatographic and immunological methods. / Ellegaard, Pernille K; Poulsen, Henrik E.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 76, No. 2, 2016, p. 151-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ellegaard, PK & Poulsen, HE 2016, 'Tobacco smoking and oxidative stress to DNA: a meta-analysis of studies using chromatographic and immunological methods', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 76, no. 2, pp. 151-8. https://doi.org/10.3109/00365513.2015.1127407

APA

Ellegaard, P. K., & Poulsen, H. E. (2016). Tobacco smoking and oxidative stress to DNA: a meta-analysis of studies using chromatographic and immunological methods. Scandinavian Journal of Clinical & Laboratory Investigation, 76(2), 151-8. https://doi.org/10.3109/00365513.2015.1127407

Vancouver

Ellegaard PK, Poulsen HE. Tobacco smoking and oxidative stress to DNA: a meta-analysis of studies using chromatographic and immunological methods. Scandinavian Journal of Clinical & Laboratory Investigation. 2016;76(2):151-8. https://doi.org/10.3109/00365513.2015.1127407

Author

Ellegaard, Pernille K ; Poulsen, Henrik E. / Tobacco smoking and oxidative stress to DNA : a meta-analysis of studies using chromatographic and immunological methods. In: Scandinavian Journal of Clinical & Laboratory Investigation. 2016 ; Vol. 76, No. 2. pp. 151-8.

Bibtex

@article{5706f6ed2814453c974cffe440452dc9,
title = "Tobacco smoking and oxidative stress to DNA: a meta-analysis of studies using chromatographic and immunological methods",
abstract = "Oxidative stress to DNA from smoking was investigated in one randomized smoking cessation study and in 36 cohort studies from excretion of urinary 8-oxo-7-hydrodeoxyguanosine (8-oxodG). Meta-analysis of the 36 cohort studies showed smoking associated with a 15.7% (95% CL 11.0:20.3, p < 0.0001) increased oxidative stress to DNA, in agreement with the reduction of oxidative stress to DNA found in the smoking cessation study. Meta-analysis of the 22 studies that used chromatography methodology on 1709 persons showed a significant 29.3% increase in smokers (95% CL 17.3;41.3), but meta-analysis of 14 studies on 3668 persons using ELISA methodology showed a non-significant effect of 8.7% [95% CL -1.2;18.6]. Tobacco smoke induces oxidative damage to DNA; however, this is not detected with ELISA methodology. Currently, the use of existing ELISA methodology to measure urinary excretion of 8-oxo-7-hydrodeoxyguanosine cannot be recommended.",
keywords = "Journal Article, Research Support, Non-U.S. Gov't",
author = "Ellegaard, {Pernille K} and Poulsen, {Henrik E}",
year = "2016",
doi = "10.3109/00365513.2015.1127407",
language = "English",
volume = "76",
pages = "151--8",
journal = "Scandinavian Journal of Clinical & Laboratory Investigation",
issn = "0036-5513",
publisher = "Taylor & Francis",
number = "2",

}

RIS

TY - JOUR

T1 - Tobacco smoking and oxidative stress to DNA

T2 - a meta-analysis of studies using chromatographic and immunological methods

AU - Ellegaard, Pernille K

AU - Poulsen, Henrik E

PY - 2016

Y1 - 2016

N2 - Oxidative stress to DNA from smoking was investigated in one randomized smoking cessation study and in 36 cohort studies from excretion of urinary 8-oxo-7-hydrodeoxyguanosine (8-oxodG). Meta-analysis of the 36 cohort studies showed smoking associated with a 15.7% (95% CL 11.0:20.3, p < 0.0001) increased oxidative stress to DNA, in agreement with the reduction of oxidative stress to DNA found in the smoking cessation study. Meta-analysis of the 22 studies that used chromatography methodology on 1709 persons showed a significant 29.3% increase in smokers (95% CL 17.3;41.3), but meta-analysis of 14 studies on 3668 persons using ELISA methodology showed a non-significant effect of 8.7% [95% CL -1.2;18.6]. Tobacco smoke induces oxidative damage to DNA; however, this is not detected with ELISA methodology. Currently, the use of existing ELISA methodology to measure urinary excretion of 8-oxo-7-hydrodeoxyguanosine cannot be recommended.

AB - Oxidative stress to DNA from smoking was investigated in one randomized smoking cessation study and in 36 cohort studies from excretion of urinary 8-oxo-7-hydrodeoxyguanosine (8-oxodG). Meta-analysis of the 36 cohort studies showed smoking associated with a 15.7% (95% CL 11.0:20.3, p < 0.0001) increased oxidative stress to DNA, in agreement with the reduction of oxidative stress to DNA found in the smoking cessation study. Meta-analysis of the 22 studies that used chromatography methodology on 1709 persons showed a significant 29.3% increase in smokers (95% CL 17.3;41.3), but meta-analysis of 14 studies on 3668 persons using ELISA methodology showed a non-significant effect of 8.7% [95% CL -1.2;18.6]. Tobacco smoke induces oxidative damage to DNA; however, this is not detected with ELISA methodology. Currently, the use of existing ELISA methodology to measure urinary excretion of 8-oxo-7-hydrodeoxyguanosine cannot be recommended.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.3109/00365513.2015.1127407

DO - 10.3109/00365513.2015.1127407

M3 - Journal article

C2 - 26767849

VL - 76

SP - 151

EP - 158

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 0036-5513

IS - 2

ER -

ID: 164614679