Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus
Research output: Contribution to journal › Journal article › Research › peer-review
Plasminogen activators (PAs) proteolytically convert plasminogen to plasmin, which, in turn, can degrade most proteins. This system has been implicated in a variety of biological processes. Using immunocytochemical methods, we here describe the localization of tissue-type PA (t-PA) in rat somatostatin cells. In the pancreatic islets, a low number of strongly t-PA-immunoreactive cells was found. By sequential staining, we found these cells to constitute a subpopulation of the somatostatin cells. Biochemical analysis of extracts from isolated rat islets demonstrated the presence of t-PA, and immunoblotting analysis demonstrated one band with a similar electrophoretic mobility. No urokinase-type PA immunoreactivity was found in the rat endocrine pancreas. A granular t-PA immunoreactivity resembling that found in adjacent sections with somatostatin antiserum was found in the median eminence of the hypothalamus. No t-PA immunoreactivity could be detected in somatostatin cells of the gastric and intestinal mucosa.
Original language | English |
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Journal | Endocrinology |
Volume | 121 |
Issue number | 6 |
Pages (from-to) | 2238-44 |
Number of pages | 7 |
ISSN | 0013-7227 |
Publication status | Published - Dec 1987 |
- Animals, Hypothalamus, Immunoenzyme Techniques, Islets of Langerhans, Male, Median Eminence, Molecular Weight, Rats, Rats, Inbred Strains, Somatostatin, Tissue Plasminogen Activator
Research areas
ID: 47974815