Three cDNA clones for barley leaf acyl carrier proteins I and III
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Three cDNA clones for barley leaf acyl carrier proteins I and III. / Hansen, Lars.
In: Carlsberg Research Communications, Vol. 52, No. 6, 11.1987, p. 381-392.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Three cDNA clones for barley leaf acyl carrier proteins I and III
AU - Hansen, Lars
PY - 1987/11
Y1 - 1987/11
N2 - Three cDNA clones for acyl carrier protein (ACP) isolated from a λgt11 cDNA library prepared from greening barley seedling leaves using a 66'mer oligonucleotide probe have been sequenced. pACP 2 (371 bases) and pACP 11 (768 bases) code for the 90 amino acid chloroplast located ACP I. pACP 1 (520 bases) codes for a hitherto unknown protein with 83 amino acids that has been designated ACP III. The primary structure of its 27 amino acid partial transit peptide bears no obvious similarities to the transit peptide of ACP I. Northern analysis of immature caryopses' poly A+ RNA using the 66'mer probe reveals the presence of an ACP mRNA of significantly smaller size than the ACP mRNAs detectable in the leaf.
AB - Three cDNA clones for acyl carrier protein (ACP) isolated from a λgt11 cDNA library prepared from greening barley seedling leaves using a 66'mer oligonucleotide probe have been sequenced. pACP 2 (371 bases) and pACP 11 (768 bases) code for the 90 amino acid chloroplast located ACP I. pACP 1 (520 bases) codes for a hitherto unknown protein with 83 amino acids that has been designated ACP III. The primary structure of its 27 amino acid partial transit peptide bears no obvious similarities to the transit peptide of ACP I. Northern analysis of immature caryopses' poly A+ RNA using the 66'mer probe reveals the presence of an ACP mRNA of significantly smaller size than the ACP mRNAs detectable in the leaf.
KW - Fatty acid synthetase
KW - nuclear coded chloroplast protein
KW - sequence homology
KW - transit peptide
UR - http://www.scopus.com/inward/record.url?scp=0040471704&partnerID=8YFLogxK
U2 - 10.1007/BF02907526
DO - 10.1007/BF02907526
M3 - Journal article
AN - SCOPUS:0040471704
VL - 52
SP - 381
EP - 392
JO - Carlsberg Research Communications
JF - Carlsberg Research Communications
SN - 0105-1938
IS - 6
ER -
ID: 309202910