The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress. / Clausing, Emanuel; Mayer, Andreas; Chanarat, Sittinan; Müller, Barbara; Germann, Susanne M; Cramer, Patrick; Lisby, Michael; Strässer, Katja.

In: Journal of Biological Chemistry, Vol. 285, No. 53, 31.12.2010, p. 41665-41674.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Clausing, E, Mayer, A, Chanarat, S, Müller, B, Germann, SM, Cramer, P, Lisby, M & Strässer, K 2010, 'The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress', Journal of Biological Chemistry, vol. 285, no. 53, pp. 41665-41674. https://doi.org/10.1074/jbc.M110.193292

APA

Clausing, E., Mayer, A., Chanarat, S., Müller, B., Germann, S. M., Cramer, P., Lisby, M., & Strässer, K. (2010). The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress. Journal of Biological Chemistry, 285(53), 41665-41674. https://doi.org/10.1074/jbc.M110.193292

Vancouver

Clausing E, Mayer A, Chanarat S, Müller B, Germann SM, Cramer P et al. The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress. Journal of Biological Chemistry. 2010 Dec 31;285(53):41665-41674. https://doi.org/10.1074/jbc.M110.193292

Author

Clausing, Emanuel ; Mayer, Andreas ; Chanarat, Sittinan ; Müller, Barbara ; Germann, Susanne M ; Cramer, Patrick ; Lisby, Michael ; Strässer, Katja. / The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 53. pp. 41665-41674.

Bibtex

@article{4bd36f0390b741eaaca5713f7ff4e79a,
title = "The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress",
abstract = "Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNA-binding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-¿C mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52 foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress.",
keywords = "Alleles, Cyclin-Dependent Kinases, Cyclins, DNA Damage, DNA Replication, Genome, Genome-Wide Association Study, Microscopy, Fluorescence, Mutation, Oligonucleotide Array Sequence Analysis, Protein Interaction Mapping, Protein Structure, Tertiary, Recombination, Genetic, Replication Protein A, Saccharomyces cerevisiae Proteins, Temperature",
author = "Emanuel Clausing and Andreas Mayer and Sittinan Chanarat and Barbara M{\"u}ller and Germann, {Susanne M} and Patrick Cramer and Michael Lisby and Katja Str{\"a}sser",
year = "2010",
month = dec,
day = "31",
doi = "10.1074/jbc.M110.193292",
language = "English",
volume = "285",
pages = "41665--41674",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "53",

}

RIS

TY - JOUR

T1 - The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress

AU - Clausing, Emanuel

AU - Mayer, Andreas

AU - Chanarat, Sittinan

AU - Müller, Barbara

AU - Germann, Susanne M

AU - Cramer, Patrick

AU - Lisby, Michael

AU - Strässer, Katja

PY - 2010/12/31

Y1 - 2010/12/31

N2 - Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNA-binding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-¿C mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52 foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress.

AB - Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNA-binding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-¿C mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52 foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress.

KW - Alleles

KW - Cyclin-Dependent Kinases

KW - Cyclins

KW - DNA Damage

KW - DNA Replication

KW - Genome

KW - Genome-Wide Association Study

KW - Microscopy, Fluorescence

KW - Mutation

KW - Oligonucleotide Array Sequence Analysis

KW - Protein Interaction Mapping

KW - Protein Structure, Tertiary

KW - Recombination, Genetic

KW - Replication Protein A

KW - Saccharomyces cerevisiae Proteins

KW - Temperature

U2 - 10.1074/jbc.M110.193292

DO - 10.1074/jbc.M110.193292

M3 - Journal article

C2 - 21075850

VL - 285

SP - 41665

EP - 41674

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 53

ER -

ID: 33764747