The T-cell accessory molecule CD4 recognizes a monomorphic determinant on isolated Ia
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The T-cell accessory molecule CD4 recognizes a monomorphic determinant on isolated Ia. / Gay, D; Buus, S; Pasternak, J; Kappler, J; Marrack, P.
In: Proceedings of the National Academy of Science of the United States of America, Vol. 85, No. 15, 1988, p. 5629-33.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The T-cell accessory molecule CD4 recognizes a monomorphic determinant on isolated Ia
AU - Gay, D
AU - Buus, S
AU - Pasternak, J
AU - Kappler, J
AU - Marrack, P
N1 - Keywords: Animals; Antigens, Differentiation, T-Lymphocyte; Cell Membrane; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Histocompatibility Antigens Class II; Hybridomas; Ligands; Liposomes; Microspheres; Receptors, Antigen, T-Cell; T-Lymphocytes
PY - 1988
Y1 - 1988
N2 - The membrane protein CD4 is commonly found on mature T cells specific for antigen in association with class II major histocompatibility complex (MHC; Ia) proteins. This correlation has led to the suggestion that CD4 binds to a monomorphic region of the Ia molecule on the antigen-presenting cell (APC) and functions either by enhancing interaction between the T cell and the APC, or conversely, by transducing negative signals to the T cell. To address this hypothesis, we have made use of sublines from an unusual T hybrid that is class I MHC restricted but also CD4+. By incorporating purified MHC proteins into a planar membrane system, we show that different Ia molecules can greatly enhance the ability of a CD4+ but not a CD4- variant of this class I-restricted T hybrid to respond to isolated class I molecules. T-cell responses can be strongly augmented by the concurrent expression of CD4 on the T cell and any of four different Ia proteins on planar membranes, thus supporting the idea that CD4 binds to a monomorphic region of the Ia molecule and increases the avidity with which the T cell can interact with its target.
AB - The membrane protein CD4 is commonly found on mature T cells specific for antigen in association with class II major histocompatibility complex (MHC; Ia) proteins. This correlation has led to the suggestion that CD4 binds to a monomorphic region of the Ia molecule on the antigen-presenting cell (APC) and functions either by enhancing interaction between the T cell and the APC, or conversely, by transducing negative signals to the T cell. To address this hypothesis, we have made use of sublines from an unusual T hybrid that is class I MHC restricted but also CD4+. By incorporating purified MHC proteins into a planar membrane system, we show that different Ia molecules can greatly enhance the ability of a CD4+ but not a CD4- variant of this class I-restricted T hybrid to respond to isolated class I molecules. T-cell responses can be strongly augmented by the concurrent expression of CD4 on the T cell and any of four different Ia proteins on planar membranes, thus supporting the idea that CD4 binds to a monomorphic region of the Ia molecule and increases the avidity with which the T cell can interact with its target.
M3 - Journal article
C2 - 3261012
VL - 85
SP - 5629
EP - 5633
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 15
ER -
ID: 9947434