The Scottish Structural Proteomics Facility: targets, methods and outputs
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The Scottish Structural Proteomics Facility: targets, methods and outputs. / Oke, Muse; Carter, Lester G; Johnson, Kenneth A; Liu, Huanting; McMahon, Stephen A; Yan, Xuan; Kerou, Melina; Weikart, Nadine D; Kadi, Nadia; Sheikh, Md Arif; Schmelz, Stefan; Dorward, Mark; Zawadzki, Michal; Cozens, Christopher; Falconer, Helen; Powers, Helen; Overton, Ian M; van Niekerk, C A Johannes; Peng, Xu; Patel, Prakash; Garrett, Roger Antony; Prangishvili, David; Botting, Catherine H; Coote, Peter J; Dryden, David T F; Barton, Geoffrey J; Schwarz-Linek, Ulrich; Challis, Gregory L; Taylor, Garry L; White, Malcolm F; Naismith, James H.
In: Journal of Structural and Functional Genomics, Vol. 11, No. 2, 01.06.2010, p. 167-80.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The Scottish Structural Proteomics Facility: targets, methods and outputs
AU - Oke, Muse
AU - Carter, Lester G
AU - Johnson, Kenneth A
AU - Liu, Huanting
AU - McMahon, Stephen A
AU - Yan, Xuan
AU - Kerou, Melina
AU - Weikart, Nadine D
AU - Kadi, Nadia
AU - Sheikh, Md Arif
AU - Schmelz, Stefan
AU - Dorward, Mark
AU - Zawadzki, Michal
AU - Cozens, Christopher
AU - Falconer, Helen
AU - Powers, Helen
AU - Overton, Ian M
AU - van Niekerk, C A Johannes
AU - Peng, Xu
AU - Patel, Prakash
AU - Garrett, Roger Antony
AU - Prangishvili, David
AU - Botting, Catherine H
AU - Coote, Peter J
AU - Dryden, David T F
AU - Barton, Geoffrey J
AU - Schwarz-Linek, Ulrich
AU - Challis, Gregory L
AU - Taylor, Garry L
AU - White, Malcolm F
AU - Naismith, James H
PY - 2010/6/1
Y1 - 2010/6/1
N2 - The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.
AB - The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.
KW - Computational Biology
KW - Crystallization
KW - Humans
KW - Laboratories
KW - Proteins
KW - Proteomics
KW - Scotland
U2 - 10.1007/s10969-010-9090-y
DO - 10.1007/s10969-010-9090-y
M3 - Journal article
C2 - 20419351
VL - 11
SP - 167
EP - 180
JO - Journal of Structural and Functional Genomics
JF - Journal of Structural and Functional Genomics
SN - 1345-711X
IS - 2
ER -
ID: 33493801