The role of dam methyltransferase in the control of DNA replication in E. coli
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The role of dam methyltransferase in the control of DNA replication in E. coli. / Boye, E; Løbner-Olesen, A.
In: Cell, Vol. 62, No. 5, 07.09.1990, p. 981-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The role of dam methyltransferase in the control of DNA replication in E. coli
AU - Boye, E
AU - Løbner-Olesen, A
PY - 1990/9/7
Y1 - 1990/9/7
N2 - The timing and control of initiation of DNA replication in E. coli was studied under conditions where the cellular level of dam methyltransferase was controlled by a temperature-inducible promoter. Flow cytometry was used to demonstrate that the synchrony of initiation at the several origins within each cell was critically dependent on the level of dam methyltransferase. Initiations were shown to be synchronous only in a narrow temperature range. The data are explained by a model where a newly replicated and therefore hemimethylated oriC is inert for reinitiation. Such a model may be applicable to eukaryotic cells, where classes of origins are initiated in synchrony and only once per cell cycle.
AB - The timing and control of initiation of DNA replication in E. coli was studied under conditions where the cellular level of dam methyltransferase was controlled by a temperature-inducible promoter. Flow cytometry was used to demonstrate that the synchrony of initiation at the several origins within each cell was critically dependent on the level of dam methyltransferase. Initiations were shown to be synchronous only in a narrow temperature range. The data are explained by a model where a newly replicated and therefore hemimethylated oriC is inert for reinitiation. Such a model may be applicable to eukaryotic cells, where classes of origins are initiated in synchrony and only once per cell cycle.
KW - DNA Replication
KW - DNA, Bacterial/biosynthesis
KW - Escherichia coli/enzymology
KW - Escherichia coli Proteins
KW - Kinetics
KW - Methyltransferases/genetics
KW - Phenotype
KW - Promoter Regions, Genetic
KW - SOS Response (Genetics)
KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)
KW - Temperature
M3 - Journal article
C2 - 2203541
VL - 62
SP - 981
EP - 989
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -
ID: 200973288